Methods for determining the coat color genotype of a pig
AssigneeDalgety PLC
InventorsLeif Andersson, Maria Johansson Moller, Richard Wales, Kenneth William Siggens, Graham Stuart Plastow
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AssigneeDalgety PLC
InventorsLeif Andersson, Maria Johansson Moller, Richard Wales, Kenneth William Siggens, Graham Stuart Plastow
Estimating the term…
Coat color is important to the pig breeding industry for a number of reasons. It is therefore desirable to develop populations of pigs which will breed true for coat color. However, establishing such populations would be time-consuming and costly using traditional test mating programs. It is therefore desirable to determine the coat color genotype of individual pigs. The inventors have shown that the KIT gene in pigs is involved with coat color determination. Specifically, the inventors have discovered that the difference between the I, IP, and i alleles of the coat color determining gene is duplication of at least part of the KIT gene in the I and IP alleles. Further, the inventors have discovered that the difference between the I and IP alleles is that, although both I and IP have a duplication in the KIT gene, only I and not IP exhibits a deletion in one of the duplicated regions. These discoveries have allowed the inventors to develop methods for distinguishing between the alleles I, IP, and i, thereby determining the genotype of individual pigs with respect to coat color. In accordance with the present invention, there are provided methods for determining the genotype of individual pigs with respect to coat color and kits for use in carrying out such methods.
A screening method to determine the presence or absence of duplication of all or part of a KIT gene to distinguish coat color genotype of a pig which comprises the steps of:
A kit for determining the coat color genotype of a pig, wherein said kit is adapted to be used with a sample of pig nucleic acid and said kit comprises (a) one or more reagents for carrying out PCR, said regents being capable of indicating the presence of duplication of all or part of the KIT sequence and (b) at least one pair of primers.
A screening method to identify the presence or absence of a deletion in a KIT gene to characterize the coat color genotype of a pig comprising the steps of:
A kit for use in determining the coat color genotype of a pig, wherein said kit is adapted to be used with a sample of pig nucleic acid and said kit comprises (a) one or more reagents for performing polymerase chain reactions, said reagents being capable of indicating the presence or absence of a deletion in the KIT sequence and (b) at least one pair of primers.
Dependencies are inferred from the claim text.