Reagents and methods useful for detecting diseases of the breast

A method of detecting mRNA of BU101 in a test sample, comprising:

• (a) performing reverse transcription on the test sample with at least one BU101 oligonucleotide primer in order to produce cDNA;
• (b) amplifying the cDNA obtained from step (a) using BU101 oligonucleotides as sense and antisense primers to obtain BU101 amplicon;
• and
• (c) detecting the presence of said BU101 amplicon in the test sample, wherein said BU101 oligonucleotides utilized in steps (a) and (b) consist of nucleic acid sequences selected from the group consisting of (I) SEQUENCE ID NO:
• 1;
• (ii) SEQUENCE ID NO:
• 3;
• (iii) SEQUENCE ID NO:
• 4;
• and (iv) exact complements of (I), (ii) or (iii).

A method of detecting a target BU101 polynucleotide in a test sample suspected of containing said target, comprising:

• (a) contacting said test sample with at least one BU101 oligonucleotide as a sense primer and with at least one BU101 oligonucleotide as an anti-sense primer and amplifying to obtain a first stage reaction product;
• (b) contacting said first stage reaction product with at least one other BU101 oligonucleotide to obtain a second stage reaction product;
• (c) detecting said second stage reaction product as an indication of the presence of the target BU101 polynucleotide, wherein the BU101 oligonucleotides utilized in steps (a) and (b) consist of nucleic acid sequences selected from the group consisting of (I) SEQUENCE ID NO:
• 1;
• (ii) SEQUENCE ID NO:
• 3;
• (iii) SEQUENCE ID NO:
• 4;
• and (iv) exact complements of (I), (ii) or (iii).

A test kit useful for detecting BU101 polynucleotide in a test sample, comprising a container containing at least one BU101 polynucleotide, wherein said polynucleotide consists of a nucleic acid selected from the group consisting of (I) SEQUENCE ID NO:

• 1;
• (ii) SEQUENCE ID NO:
• 3;
• (iii) SEQUENCE ID NO:
• 4;
• and (v) (iV) exact complements of (I), (ii) or (iii).

A purified polynucleotide, wherein said polynucleotide consists of a nucleic acid selected from the group consisting of (I) SEQUENCE ID NO:

• 1;
• (ii) SEQUENCE ID NO:
• 3;
• (iii) SEQUENCE ID NO:
• 4;
• and (iv) exact complements of (I), (ii), or (iii).

A vector comprising an insert consisting of a nucleic acid sequence derived from BU101, wherein said nucleic acid sequence is operably linked to a control sequence compatible with a desired host, and wherein said nucleic acid sequence consists of a nucleic acid (is) selected from the group consisting of (I) SEQUENCE ID NO:

• 1;
• (ii) SEQUENCE ID NO:
• 3;
• (iii) SEQUENCE ID NO:
• 4;
• and (iv) exact complements of (I), (ii), or (iii).

A composition of matter comprising an isolated BU101 polynucleotide, wherein said polynucleotide consists of a nucleic acid molecule (is) selected from the group consisting of (I) SEQUENCE ID NO:

• 1;
• (ii) SEQUENCE ID NO:
• 3;
• (iii) SEQUENCE ID NO:
• 4;
• and (iv) exact complements of (I), (ii), or (iii).