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US 6,183,754 B2Granted
Anticytomegalovirus monoclonal antibodies and processes for the in vitro diagnosis of infections by human cytomegaloviruses and a protein-kinase inducible by cytomegaloviruses and recognizable by aforesaid monoclonal antibodies
AssigneeInstitut Pasteur
InventorsFlorian Horaud, Susan Michelson, Octavian Barzu, Andre Boue, Clairo Amadei
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Abstract
The invention relates to a process for in vitro diagnosis of an infection by human cytomegaloviruses. The process consists of contacting cells, possibly carrying the infection, with a monoclonal antibody reacting with a polypeptide of molecular weight 68,000, induced by human cytomagalovirus and which possesses a protein-kinase activity. The detection of the reaction is preferably carried out by immunofluorescence.
Claims5
A purified polypeptide of viral origin inducible in human cells by human cytomegalovirus having:
- a molecular weight of about 68,000 daltons;
- is recognized by the monoclonal antibody produced in the hybridoma deposited in the C.N.C.M. (National Collection of Microorganisms and Cultures) under accession No. I-289;
- a protein-kinase activity of the casein-kinase II type;
- a capability of causing the transfer of phosphorus from ATP to casein, phosvitin, glycogen-synthetase, histones and alphaphosphorylases;
- a protein-kinase, activity which is inhibited by quercitin;
- a capability of being autophosphorylated in vitro;
- and a loss of activity at −70° C. and at 100° C., wherein said polypeptide of viral origin which is inducible in human cells by human cytomegalovirus persists throughout the infectious cycle.
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