Immunogenic peptides from the HPV E7 protein
AssigneeZycos Inc.
InventorsRobert G. Urban, Roman M. Chicz, Edward J. Collins, Mary Lynne Hedley
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Abstract
The invention provides immunogenic peptides from the HPV type 16 E7 protein that comprise overlapping class I restricted T cell epitopes. Also disclosed are methods of administering DNA molecules encoding these peptides to a host mammal.
Claims81
A method of making a polypeptide, which method comprises maintaining a cell containing a plasmid comprising a coding sequence coding for expression of a polypeptide under conditions permitting expression of said polypeptide, the polypeptide comprising a first peptide and a second peptide linked by a peptide bond, the first peptide being a peptide which controls intracellular trafficking of a peptide to which it is attached, and the second peptide consisting of a sequence 12-18 amino acids in length comprising the sequence Leu Met Gly Thr Leu Gly Ile Val Cys Pro Ile Cys (SEQ ID NO:16).
A method of making a polypeptide, which method comprises maintaining a cell containing a plasmid comprising a coding sequence coding for expression of a polypeptide under conditions permitting expression of said polypeptide, the polypeptide comprising a first peptide and a second peptide linked by a peptide bond, the first peptide being a peptide which controls intracellular trafficking of a peptide to which it is attached, and the second peptide consisting of a sequence 8-18 amino acids in length comprising the sequence Thr Leu Gly Ile Val Cys Pro Ile (SEQ ID NO:20).
A method of inducing an immune response in a mammal, which method comprises administering to a mammal a nucleic acid comprising a coding sequence coding for expression of a polypeptide comprising a first peptide and a second peptide linked by a peptide bond, the first peptide being a peptide which controls intracellular trafficking of a peptide to which it is attached, and the second peptide consisting of a sequence 12-18 amino acids in length comprising the sequence Leu Met Gly Thr Leu Gly Ile Val Cys Pro Ile Cys (SEQ ID NO:20).
A method of inducing an immune response in a mammal, which method comprises administering to a mammal a nucleic acid comprising a coding sequence coding for expression of a polypeptide comprising a first peptide and a second peptide linked by a peptide bond, the first peptide being a peptide which controls intracellular trafficking of a peptide to which it is attached, and the second peptide consisting of a sequence 8-18 amino acids in length comprising the sequence Thr Leu Gly Ile Val Cys Pro Ile (SEQ ID NO:20).
A method of inducing an immune response in a mammal, which method comprises administering to a mammal a plasmid comprising a coding sequence coding for expression of a polypeptide comprising a first peptide and a second peptide linked by a peptide bond, the first peptide being a peptide which controls intracellular trafficking of a peptide to which it is attached, and the second peptide consisting of a sequence 12-18 amino acids in length comprising the sequence Leu Met Gly Thr Leu Gly Ile Val Cys Pro Ile Cys (SEQ ID NO:16).
A method of inducing an immune response in a mammal, which method comprises administering to a mammal a plasmid comprising a coding sequence coding for expression of a polypeptide comprising a first peptide and a second peptide linked by a peptide bond, the first peptide being a peptide which controls intracellular trafficking of a peptide to which it is attached, and the second peptide consisting of a sequence 8-18 amino acids in length comprising the sequence Thr Leu Gly Ile Val Cys Pro Ile (SEQ ID NO:20).
A method of inducing an immune response in a mammal, which method comprises administering to a mammal a microparticle comprising
- (a) a polymeric matrix;
- and
- (b) a plasmid comprising a coding sequence coding for expression of a polypeptide comprising a first peptide and a second peptide linked by a peptide bond, the first peptide being a peptide which controls intracellular trafficking of a peptide to which it is attached, and the second peptide consisting of a sequence 12-18 amino acids in length comprising the sequence Leu Met Gly Thr Leu Gly Ile Val Cys Pro Ile Cys (SEQ ID NO:16).
A method of inducing an immune response in a mammal, which method comprises administering to a mammal a microparticle comprising
- (a) a polymeric matrix;
- and
- (b) a plasmid comprising a coding sequence coding for expression of a polypeptide comprising a first peptide and a second peptide linked by a particle bond, the first peptide being a peptide which controls intracellular trafficking of a peptide to which it is attached, and the second peptide consisting of a sequence 8-18 amino acids in length comprising the sequence Thr Leu Gly Ile Val Cys Pro Ile (SEQ ID NO:20).
A method of inducing a cell mediated, anti-HPV immune response in a mammal, which method comprises administering to the mammal a DNA comprising the sequence of SEQ ID NO:7.
A method of inducing an immune response in a patient, which method comprises administering to the patient a microparticle having a diameter of less than 20 microns and consisting essentially of a polymeric matrix and a nucleic acid molecule, wherein the polymeric matrix consists essentially of PLGA and the nucleic acid molecule comprises the sequence of SEQ ID NO:7.
A method of inducing an immune response in an mammal, which method comprises administering a nucleic acid comprising a coding sequence coding for expression of a peptide less than 19 amino acids in length, wherein the peptide comprises the amino acid sequence Leu Met Gly Thr Leu Gly Ile Val Cys Pro Ile Cys (SEQ ID NO:16).
A method of inducing an immune response in an mammal, which method comprises administering a nucleic acid comprising a coding sequence coding for expression of a peptide less than 19 amino acids in length, wherein the peptide comprises the amino acid sequence Gly Thr Leu Gly Ile Val Cys Pro Ile (SEQ ID NO:21).
A method of inducing a cell-mediated, anti-HPV immune response in a mammal, which method comprises administering to the mammal a DNA comprising the sequence of SEQ ID NO:5.
A method of inducing an immune response in a patient, which method comprises administering to the patient a microparticle having a diameter of less than 20 microns and consisting essentially of a polymeric matrix and a nucleic acid molecule, wherein the polymeric matrix consists essentially of PLGA and the nucleic acid molecule comprises the sequence of SEQ ID NO:5.
A method of inducing a cell-mediated, anti-HPV immune response in a mammal, which method comprises administering to the mammal a DNA comprising the sequence of nucleotides 3290-3413 of SEQ ID NO:7.
A method of inducing an immune response in a patient, which method comprises administering to the patient a microparticle having a diameter of less than 20 microns and consisting essentially of a polymeric matrix and a nucleic acid molecule, wherein the polymeric matrix consists essentially of PLGA and the nucleic acid molecule comprises the sequence nucleotides 3290-3413 of SEQ ID NO:7.
Dependencies are inferred from the claim text.