Maize promoters
AssigneePioneer Hi-Bred International, Inc.
InventorDouglas A. Rice
Life of this patent
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Abstract
The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for constitutive promoters isolated from maize genes encoding histone H2B, metallothionein, alpha-tubulin 3, elongation factor efla, ribosomal protein rps8, chlorophyll a/b binding protein, and glyceraldehyde-3-phosphate dehydrogenase. A method for constitutively expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the constitutive promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.
Claims31
An isolated nucleic acid molecule having a nucleotide sequence for a promoter that is capable of initiating transcription in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
- 1, 2,3,4,5,6,7, or10;
- b) a nucleotide sequence selected from the group consisting of sequences deposited as ATCC Accession Nos. 207123, 207120, 207125, 207121, 207122, 207124, 207126, and 207119;
- c) a nucleotide sequence comprising at least 40 contiguous nucleotides of the sequence set forth in SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, or 10;
- and
- d) a nucleotide sequence that has at least about 70% sequence identity to a sequence set forth in a) or b).
An isolated nucleic acid molecule having a nucleotide sequence for a promoter that is capable of initiating transcription in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
- 8;
- b) the nucleotide sequence deposited as ATCC Accession No. 207127;
- c) a nucleotide sequence comprising at least 40 contiguous nucleotides of the C-terminal end of the sequence set forth in SEQ ID NO:
- 8, wherein said C-terminal end comprises nucleotides 272-467 of SEQ ID NO:
- 8;
- and
- d) a nucleotide sequence that has at least about 70% sequence identity to a sequence set forth in a) or b).
An isolated nucleic acid molecule having a nucleotide sequence for a promoter that is capable of initiating transcription in a plant cell, wherein said nucleotide sequence is selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
- 9;
- b) the nucleotide sequence deposited as ATCC Accession No. 207128;
- c) a nucleotide sequence comprising at least 40 contiguous nucleotides of the C-terminal end of the sequence set forth in SEQ ID NO:
- 9, wherein said C-terminal end comprises nucleotides 86-467 of SEQ ID NO:
- 9;
- and
- d) a nucleotide sequence that has at least about 70% sequence identity to a sequence set forth in a) or b).
A method for expressing a heterologous nucleotide sequence in a plant, said method comprising transforming a plant cell with a DNA construct comprising said heterologous nucleotide sequence operably linked to a promoter that is capable of initiating transcription in a plant cell and regenerating a stably transformed plant from said plant cell, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, or 10;
- b) a nucleotide sequence selected from the group consisting of sequences deposited as ATCC Accession Nos. 297123, 207120, 207125, 207121, 207122, 207124, 207126, and 207119;
- c) a nucleotide sequence comprising at least 40 contiguous nucleotides of the sequence set forth in SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, or 10;
- and
- d) a nucleotide sequence that has at least about 70% sequence identity to a sequence set forth in a) or b).
A method for expressing a heterologous nucleotide sequence in a plant, said method comprising transforming a plant cell with a DNA construct comprising said heterologous nucleotide sequence operably linked to a promoter that is capable of initiating transcription in a plant cell and regenerating a stably transformed plant from said plant cell, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
- 8;
- b) the nucleotide sequence deposited as ATCC Accession No. 207127;
- c) a nucleotide sequence comprising at least 40 contiguous nucleotides of the C-terminal end of the sequence set forth in SEQ ID NO:
- 8, wherein said C-terminal end comprises nucleotides 272-467 of SEQ ID NO:
- 8;
- and
- d) a nucleotide sequence that has at least about 70% sequence identity to a sequence set forth in a) or b).
A method for expressing a heterologous nucleotide sequence in a plant, said method comprising transforming a plant cell with a DNA construct comprising said heterologous nucleotide sequence operably linked to a promoter that is capable of initiating transcription in a plant cell and regenerating a stably transformed plant from said plant cell, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
- 9;
- b) the nucleotide sequence deposited as ATCC Accession No. 207128;
- c) a nucleotide sequence comprising at least 40 contiguous nucleotides of the C-terminal end of the sequence set forth in SEQ ID NO:
- 9, wherein said C-terminal end comprises nucleotides 86-467 of SEQ ID NO:
- 9;
- and
- d) a nucleotide sequence that has at least about 70% sequence identity to a sequence set forth in a) or b).
A plant cell stably transformed with a DNA construct comprising a heterologous nucleotide sequence operably linked to a promoter that is capable of initiating transcription in said plant cell, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, or 10;
- b) a nucleotide sequence selected from the group consisting of sequences deposited as ATCC Accession Nos. 207123, 207120, 207125, 207121,207122, 207124, 207126, and 207119;
- c) a nucleotide sequence comprising at least 40 contiguous nucleotides of the sequence set forth in SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, or 10;
- and
- d) a nucleotide sequence that has at least about 70% sequence identity to a sequence set forth in a) or b).
A plant cell stably transformed with a DNA construct comprising a heterologous nucleotide sequence operably linked to a promoter that is capable of initiating transcription in said plant cell, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
- 8;
- b) the nucleotide sequence deposited as ATCC Accession No. 207127;
- c) a nucleotide sequence comprising at least 40 contiguous nucleotides of the C-terminal end of the sequence set forth in SEQ ID NO:
- 8, wherein said C-terminal end comprises nucleotides 272-467 of SEQ ID NO:
- 8;
- and
- d) a nucleotide sequence that has at least about 70% sequence identity to a sequence set forth in a) or b).
A plant cell stably transformed with a DNA construct comprising a heterologous nucleotide sequence operably linked to a promoter that is capable of initiating transcription in said plant cell, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
- 9;
- b) the nucleotide sequence deposited as ATCC Accession No. 207128;
- c) a nucleotide sequence comprising at least 40 contiguous nucleotides of the C-terminal end of the sequence set forth in SEQ ID NO:
- 9, wherein said C-terminal end comprises nucleotides 86-467 of SEQ ID NO:
- 9;
- and
- d) a nucleotide sequence that has at least about 70% sequence identity to a sequence set forth in a) or b).
A plant stably transformed with a DNA construct comprising a heterologous nucleotide sequence operably linked to a promoter that is capable of initiating transcription in a plant cell, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
- 1,2,3,4,5,6,7, or 10;
- b) a nucleotide sequence selected from the group consisting of sequences deposited as ATCC Accession Nos. 207123, 207120, 207125, 207121, 207122, 207124, 207126, and 207119;
- c) a nucleotide sequence comprising at least 40 contiguous nucleotides of the sequence set forth in SEQ ID NO:
- 1, 2, 3, 4, 5, 6, 7, or 10;
- and
- d) a nucleotide sequence that has at least about 70% sequence identity to a sequence set forth in a) or b).
A plant stably transformed with a DNA construct comprising a heterologous nucleotide sequence operably linked to a promoter that is capable of initiating transcription in a plant cell, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
- 8;
- b) the nucleotide sequence deposited as ATCC Accession No. 207127;
- c) a nucleotide sequence comprising at least 40 contiguous nucleotides of the C-terminal end of the sequence set forth in SEQ ID NO:
- 8, wherein said C-terminal end comprises nucleotides 272-467 of SEQ ID NO:
- 8;
- and
- d) a nucleotide sequence that has at least about 70% sequence identity to a sequence set forth in a) or b).
A plant stably transformed with a DNA construct comprising a heterologous nucleotide sequence operably linked to a promoter that is capable of initiating transcription in a plant cell, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
- a) a nucleotide sequence comprising the sequence set forth in SEQ ID NO:
- 9;
- b) the nucleotide sequence deposited as ATCC Accession No. 207128;
- c) a nucleotide sequence comprising at least 40 contiguous nucleotides of the C-terminal end of the sequence set forth in SEQ ID NO:
- 9, wherein said C-terminal end comprises nucleotides 86-467 of SEQ ID NO:
- 9;
- and
- d) a nucleotide sequence that has at least about 70% sequence identity to a sequence set forth in a) or b).
Dependencies are inferred from the claim text.