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US 6,172,281 B2Granted
Recombinant plant expressing non-competitively binding BT insecticidal crystal proteins
AssigneeAventis CropScience GmbH
InventorsHerman Van Mellaert, Johan Botterman, Jeroen Van Rie, Henk Joos
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Abstract
Plants made resistant to insects by transforming their nuclear genome with two or more DNA sequences, each encoding a different non-competitively binding B. thuringiensis protoxin or insecticidal part thereof, preferably the toxin thereof.
Claims31
A method for producing a plant with increased insect resistance, comprising the steps of:
- expressing in a plant chimeric genes encoding different Bacillus thuringiensis proteins, or insecticidal portions thereof, wherein said Bacillus thuringiensis proteins, or insecticidal portions thereof, are insecticidal to the same target insect and bind without competition to different binding sites in the gut membranes of said target insect as can be determined by in vitro binding assays with brush border membrane vesicles.
A method for producing a plant with increased resistance to a target insect pest species, comprising expressing in cells of a plant two genes encoding Bacillus thuringiensis proteins, or insecticidal portions thereof,
- wherein said proteins comprise a first protein and a second protein and said second protein is chosen using the following procedure:
- a) obtaining a strain of said target insect pest species that developed resistance to said first protein or an insecticidal portion thereof,
- b) carrying out insect bioassays and competitive binding studies using said first protein and a second protein or an insecticidal portion thereof,
- c) selecting a second protein, or an insecticidal portion thereof, that remains fully insecticidal to said resistant insect strain and binds to a different binding site in the target insect gut membranes compared to the first protein, or an insecticidal portion thereof.
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Dependencies are inferred from the claim text.