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AssigneeHoechst Marion Roussel, Inc.
InventorsHerman Slijkhuis, Eric Bastiaan Smaal, Gerardus Cornelis Maria Selten
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An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins, of catalyzing an oxidation step in the biological pathway for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting of: the conversion of cholesterol to pregnenolone; the conversion of pregnenolone to progesterone; the conversion of progesterone to 17α-hydroxyprogesterone; the conversion of 17α-hydroxyprogesterone to cortexolone; the conversion of cortexolone to hydrocortisone, and the corresponding control sequences effective in said host.
An expression cassette comprising a heterologous DNA encoding two or more enzymes from the metabolic pathway for the bioconversion of cholesterol to hydrocortisone wherein one of the enzymes is a bovine enzyme which catalyzes the oxidation of progesterone to 17α-hydroxyprogesterone and the remaining one or more bovine or human enzymes catalyze at least one reaction selected from the group consisting of the oxidation of cholesterol to pregnenolone by bovine enzyme;
An expression cassette comprising a heterologous DNA encoding an enzyme from the metabolic pathway for the bioconversion of cholesterol to hydrocortisone which is a bovine enzyme which catalyzes the oxidation of progesterone to 17α-hydroxyprogesterone and further comprising one or more additional heterologous DNAs encoding one or more additional bovine or human enzymes from the metabolic pathway for the bioconversion of cholesterol to hydrocortisone, which one or more additional enzymes catalyze at least one reaction selected from the group consisting of:
A recombinant host cell and progeny thereof comprising a heterologous DNA encoding two or more enzymes from the metabolic pathway for the bioconversion of cholesterol to hydrocortisone wherein one of the enzymes is a bovine enzyme which catalyzes the oxidation of progesterone to 17α-hydroxyprogesterone and the remaining one or more bovine or human enzymes catalyzes at least one reaction selected from the group consisting of:
Dependencies are inferred from the claim text.