Mixed cell diagnostic systems
AssigneesDiagnostic Hybrids, Inc., University Hospitals of Cleveland
InventorsDavid R. Scholl, Yung T. Huang, Patricia Gail Ray Goodrum
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Abstract
The present invention generally relates to the field of diagnostic microbiology, and, more particularly, to compositions and methods for detecting and differentiating one or more viruses or other intracellular parasites present in a specimen. The present invention also provides compositions and methods to evaluate the susceptibility of a organisms to antimicrobial agents.
Claims20
A method for the detection and identification of influenza virus in a sample, comprising the steps of:
- a) providing:
- i) a sample suspected of containing influenza virus;
- and
- ii) a mixed cell culture comprising mink lung cells and a second cell type;
- b) inoculating said mixed cell culture with said sample to produce an inoculated culture;
- and
- c) observing said inoculated culture for the presence of said influenza virus.
A method for the detection and identification of enterovirus in a sample, comprising:
- a) providing:
- i) a sample suspected of containing enterovirus;
- and
- ii) a mixed cell culture comprising at least two cell types, wherein said cell types comprise a first cell type and a second cell type, and wherein said first cell type is buffalo green monkey kidney cell and said second cell type is selected from human mucoepidermoid cell and human lung carcinoma cell;
- b) inoculating said mixed cell culture with said sample to produce an inoculated culture;
- and
- c) observing said inoculated culture for the presence of said enterovirus.
A method for the detection and identification of herpes virus in a sample, comprising:
- a) providing:
- i) a sample suspected of containing herpes virus;
- and
- ii) a mixed cell culture comprising at least two cell types, wherein said cell types comprise a first cell type and a second cell type, and wherein said first cell type is MRC-5 and said second cell type is CV-1;
- b) inoculating said mixed cell culture with said sample to produce an inoculated culture;
- and
- c) observing said inoculated culture for the presence of said herpes virus.
A method for the detection and identification of a virus selected from cytomegalovirus, influenza viruses, parainfluenza viruses, respiratory syncytial virus, rhinoviruses, coronoviruses, and adenoviruses, in a sample, comprising the steps of:
- a) providing:
- i) a sample suspected of containing one or more of said viruses;
- and
- ii) a mixed cell culture comprising at least two cell types, wherein said cell types comprise a first cell type and a second cell type, and wherein said first cell type is a first genetically engineered cell type and said second cell type is a second genetically engineered cell type;
- b) inoculating said mixed cell culture with said sample to produce an inoculated culture;
- and
- c) observing said inoculated culture for the presence of one or more of said viruses.
A method for the detection and identification of a member of the genus Chlamydia in a sample, comprising:
- a) providing:
- i) a sample suspected of containing a member of the genus Chlamydia;
- and
- ii) a mixed cell culture comprising at least two cell types, wherein said cell types comprise a first cell type and a second cell type, and wherein said first cell type is McCoy cell;
- b) inoculating said mixed cell culture with said sample to produce an inoculated culture;
- and
- c) observing said inoculated culture for the presence of at least one of said member of the genus Chlamydia.
Dependencies are inferred from the claim text.