Method and device for flow-cytometric microorganism analysis

A microorganism analyzer, used with a flow cytometer that measures the particle size of microorganisms in a culture sample wherein the specimen to be measured has been added to a culture solution, comprising:

• a first measurement means for obtaining a first particle-size distribution of a culture sample after a prescribed period of culture, using optical information obtained from the scattered light detected by said flow cytometer;
• a second measurement means for obtaining a second particle-size distribution of a culture sample before culture, using optical information obtained from the scattered light detected by said flow cytometer;
• analysis means for obtaining a particle-size distribution of microorganisms cultured in said culture sample from the difference between said first particle-size distribution and said second particle-size distribution;
• and
• output means for outputting said obtained microorganism particle-size distribution.

In a computer-readable recording medium used with a flow cytometer that measures the particle size of microorganisms within a culture sample wherein the specimen to be measured has been added to a culture solution, said recording medium containing an analytical program to analyze the measurement results of said particle size, a computer-readable recording medium containing an analytical program that executes:

• (A) a stage for obtaining a first particle-size distribution of a culture sample after a prescribed period of culture, using optical information obtained from the scattered light detected by said flow cytometer;
• (B) a stage for obtaining a second particle-size distribution of the culture sample before said culture, using optical information obtained from the scattered light detected by said flow cytometer;
• (C) a stage for obtaining a particle-size distribution of microorganisms cultured in said culture sample from the difference between said first particle-size distribution and said second particle-size distribution;
• and
• (D) a stage for outputting said obtained microorganism particle-size distribution.