Biosensor device and method
AssigneePENCE and McGill University
InventorsR. Bruce Lennox, Robert S. Hodges, Randall T. Irvin
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Abstract
A biosensor apparatus for detecting a binding event between a ligand and receptor. The apparatus includes a biosensor surface and surface-bound two-subunit heterodimer complexes composed of first and second, preferably oppositely charged peptides that together form an .alpha.-helical coiled-coil heterodimer. The first peptide is attached to the biosensor surface, and the second peptide carries the ligand, accessible for binding by a ligand-binding agent. Binding of anti-ligand binding agent to the surface-bound ligand is detected by a suitable detector. A ligand-specific biosensor surface can be readily prepared from a universal template containing the first charged peptide, by addition of a selected ligand attached to the second peptide.
Claims10
A biosensor apparatus for detecting a binding event between a ligand and ligand-binding agent, comprising
- means defining a biosensor surface,
- carried on said biosensor surface, two-subunit heterodimer complexes composed of first and second peptides that together form an .alpha.-helical coiled-coil heterodimer, where said first peptide is attached to the biosensor surface,
- a ligand covalently attached to the second peptide in the two-subunit heterodimer complexes, accessible for binding by a ligand-binding agent,
- means for introducing onto the biosensor surface, an analyte selected from the group consisting of a ligand-binding agent, and a ligand or ligand analog capable of competing with a ligand-binding agent for binding to said ligand, said apparatus also including a ligand-binding agent when the analyte is a ligand or ligand analog, and
- means for detecting the binding of a ligand-binding agent to the ligand on the biosensor surface.
Dependencies are inferred from the claim text.