Plant transformation methods
AssigneeNovartis AG
InventorGenevieve Hansen
Life of this patent
Estimating the term…
Abstract
An improved method of Agrobacterium transformation of plants, particularly Gramineae, is provided, utilizing conditions capable of inhibiting Agrobacterium-induced necrosis.
Claims48
A method for transforming a plant cell or tissue with a gene construct, comprising heat shocking said plant cell or tissue before co-cultivating with Agrobacterium, wherein said heat shock treatment inhibits Agrobacterium induced necrosis in said plant cell or tissue, and said Agrobacterium comprises a vector comprising said gene construct.
A method for producing a fertile transgenic plant comprising a gene construct, which method comprises:
- (a) transforming a plant cell or tissue comprising heat shocking said plant cell or tissue before co-cultivating with Agrobacterium, wherein said heat shock treatment inhibits Agrobacterium induced necrosis in said plant cell or tissue and said Agrobacterium comprises a vector comprising said gene construct;
- and
- (b) regenerating the transformed plant cell or tissue to produce said fertile transgenic plant.
A method for transforming a maize cell or tissue with a gene construct, comprising heat shocking said maize cell or tissue before co-cultivating with Agrobacterium, wherein said heat shock treatment inhibits Agrobacterium induced necrosis in said maize cell or tissue, and said Agrobacterium comprises a vector comprising said gene construct.
A method for producing a fertile transgenic maize plant comprising a gene construct, which method comprises:
- (a) transforming a maize cell or tissue comprising heat shocking said maize cell or tissue before co-cultivating with Agrobacterium, wherein said heat shock treatment inhibits Agrobacterium induced necrosis in said maize cell or tissue, and said Agrobacterium comprises a vector comprising said gene construct;
- and
- (b) regenerating the transformed maize cell or tissue to produce said fertile transgenic maize plant.
A method for transforming a plant cell or tissue with a gene construct, comprising exposing said plant cell or tissue to Agrobacterium under conditions which inhibit Agrobacterium induced necrosis, wherein said conditions comprise delivering to or expressing in said plant cell or tissue a nucleotide sequence comprising a coding sequence of a p35, iap or dad-1 gene, said delivery or expression of said nucleotide sequence inhibits Agrobacterium induced necrosis in said plant cell or tissue, and said Agrobacterium comprises a vector comprising said gene construct.
A method for producing a fertile transgenic plant comprising a gene construct, which method comprises:
- (a) transforming a plant cell or tissue comprising exposing said plant cell or tissue to Agrobacterium under conditions which inhibit Agrobacterium induced necrosis, wherein said conditions comprise delivering to or expressing in said plant cell or tissue a nucleotide sequence comprising a coding sequence of a p35, iap or dad-1 gene, said delivery or expression of said nucleotide sequence inhibits Agrobacterium induced necrosis in said plant cell or tissue, and said Agrobacterium comprises a vector comprising said gene construct;
- and
- (b) regenerating the transformed plant cell or tissue to produce said fertile transgenic plant.
A method for transforming a maize cell or tissue with a gene construct, comprising exposing said maize cell or tissue to Agrobacterium under conditions which inhibit Agrobacterium induced necrosis, wherein said conditions comprise delivering to or expressing in said maize cell or tissue a nucleotide sequence comprising a coding sequence of a p35, iap or dad-1 gene, said delivery or expression of said nucleotide sequence inhibits Agrobacterium induced necrosis in said maize cell or tissue, and said Agrobacterium comprises a vector comprising said gene construct.
A method for producing a fertile transgenic maize plant comprising a gene construct, which method comprises:
- (a) transforming a maize cell or tissue comprising exposing said maize cell or tissue to Agrobacterium under conditions which inhibit Agrobacterium induced necrosis, wherein said conditions comprise delivering to or expressing in said maize cell or tissue a nucleotide sequence comprising a coding sequence of a p35, iap or dad-1 gene, said delivery or expression of said nucleotide sequence inhibits Agrobacterium induced necrosis in said maize cell or tissue, and said Agrobacterium comprises a vector comprising said gene construct;
- and
- (b) regenerating the transformed maize cell or tissue to produce said fertile transgenic maize plant.
A method for transforming a gramineceous plant cell or tissue with a gene construct, comprising exposing said plant cell or tissue to Agrobacterium under conditions which inhibit Agrobacterium induced necrosis, wherein said conditions comprise culturing said plant cell or tissue in a necrosis inhibiting medium, said necrosis inhibiting medium comprises (i) an ethylene inhibitor other than silver nitrate, or (ii) an ethylene synthesis inhibitor, and said Agrobacterium comprises a vector comprising said gene construct.
A method for producing a fertile transgenic gramineceous plant comprising, a gene construct, which method comprises:
- (a) transforming a gramineceous plant cell or tissue comprising exposing said plant cell or tissue to Agrobacterium under conditions which inhibit Agrobacterium induced necrosis, wherein said conditions comprise culturing said plant cell or tissue in a necrosis inhibiting medium, said necrosis inhibiting medium comprises (i) an ethylene inhibitor other than silver nitrate, or (ii) an ethylene synthesis inhibitor, and said Agrobacterium comprises a vector comprising said gene construct;
- and
- (b) regenerating the transformed plant cell or tissue to produce said fertile transgenic gramineceous plant.
A method for transforming a maize cell or tissue with a gene construct, comprising exposing said maize cell or tissue to Agrobacterium under conditions which inhibit Agrobacterium induced necrosis, wherein said conditions comprise culturing said maize cell or tissue in a necrosis inhibiting medium, said necrosis inhibiting medium comprises (i) an ethylene inhibitor other than silver nitrate, or (ii) an ethylene synthesis inhibitor, and said Agrobacterium comprises a vector comprising said gene construct.
A method for producing a fertile transgenic maize plant comprising a gene construct, which method comprises:
- (a) transforming a maize cell or tissue comprising exposing said maize cell or tissue to Agrobacterium under conditions which inhibit Agrobacterium induced necrosis, wherein said conditions comprise culturing said maize cell or tissue in a necrosis inhibiting medium, said necrosis inhibiting medium comprises (i) an ethylene inhibitor other than silver nitrate, or (ii) an ethylene synthesis inhibitor, and said Agrobacterium comprises a vector comprising said gene construct.;
- and
- (b) regenerating the transformed maize cell or tissue to produce said fertile transgenic maize plant.
A transgenic plant, plant tissue or plant cell comprising a nucleotide sequence of heterologous origin which comprises a coding sequence of a p35, iap or dad-1 gag.
A transgenic plant, plant tissue or plant cell comprising a genome having a stably integrated nucleotide sequence of heterologous origin which comprises a coding sequence of a p35, iap or dad-1 gene.
A transgenic maize plant, tissue or cell comprising a genome having a stably integrated nucleotide sequence of heterologous origin comprising a coding sequence of a p35 gene.
A transgenic maize plant, tissue or cell comprising a genome having a stably integrated nucleotide sequence of heterologous origin which comprises a coding sequence of an iap gene.
A transgenic maize plant, tissue or cell comprising a genome having a stably integrated nucleotide sequence of heterologous origin which comprises a coding sequence of a dad-1 gene.
A gramineceous plant cell or tissue culture medium, comprising
- (a) (i) an ethylene inhibitor other than silver nitrate, or (ii) an ethylene synthesis inhibitor;
- and
- (b) an Agrobacterium comprising a plasmid comprising a gene construct.
Dependencies are inferred from the claim text.