Generation of Xenogenetic antibodies

A method for producing a transgenic mouse and its progeny lacking expression of endogenous immunoglobulin light chains, comprising:

• (a) deleting the C.kappa. gene from at least one endogenous light chain locus in a mouse embryonic stem cell to prevent rearrangement of the locus and to prevent formation of a transcript of a rearranged immunoglobulin light chain locus, the deletion being effected by a targeting vector comprising a gene encoding a selectable marker;
• (b) producing from the embryonic stem cell a transgenic mouse whose somatic and germ cells contain the gene encoding the selectable marker, and
• (c) breeding the transgenic mouse as needed to produce a transgenic mouse and its progeny lacking expression of endogenous immunoglobulin light chains.

A method for producing a transgenic mouse and its progeny lacking expression of endogenous immunoglobulin light chains, comprising:

• (a) deleting the C.kappa. gene from at least one endogenous light chain locus in a mouse embryonic stem cell to prevent rearrangement of the locus and to prevent formation of a transcript of a rearranged immunoglobulin light chain locus, the deletion being effected by a targeting vector comprising a gene encoding a selectable marker;
• (b) producing from the embryonic stem cell a transgenic mouse whose somatic and germ cells contain an immunoglobulin light chain locus in which the C.kappa. gene is deleted, and (c) breeding the transgenic mouse as needed to produce a transgenic mouse and its progeny lacking expression of endogenous immunoglobulin light chains.

A method for preventing the expression of endogenous immunoglobulin light chains in a transgenic mouse and its progeny, comprising:

• (a) deleting the C.kappa. gene from at least one endogenous light chain locus in a mouse embryonic stem cell to prevent rearrangement of the locus and to prevent formation of a transcript of a rearranged immunoglobulin light chain locus, the deletion being effected by a targeting vector comprising a gene encoding a selectable marker;
• (b) producing from the embryonic stem cell a transgenic mouse whose somatic and germ cells contain the gene encoding the selectable marker, and
• (c) breeding the transgenic mouse as needed to produce a transgenic mouse and its progeny lacking expression of endogenous immunoglobulin light chains.

A method for preventing the expression of endogenous immunoglobulin light chains in a transgenic mouse and its progeny, comprising:

• (a) deleting the C.kappa. gene from at least one endogenous light chain locus in a mouse embryonic stem cell to prevent rearrangement of the locus and to prevent formation of a transcript of a rearranged immunoglobulin light chain locus, the deletion being effected by a targeting vector comprising a gene encoding a selectable marker;
• (b) producing from the embryonic stem cell a transgenic mouse whose somatic and germ cells contain an immunoglobulin light chain locus in which the C.kappa. gene is deleted, and
• (c) breeding the transgenic mouse as needed to produce a transgenic mouse and its progeny lacking expression of endogenous immunoglobulin light chains.

A method for producing a mouse embryonic stem cell with at least one inactivated endogenous immunoglobulin light chain locus, comprising deleting the C.kappa. gene from at least one endogenous immunoglobulin light chain locus in the mouse embryonic stem cell to prevent rearrangement of the locus and to prevent the formation of a transcript of a rearranged immunoglobulin light chain, the deletion being effected by a targeting vector comprising a gene encoding a selectable marker.

A method for producing a transgenic mouse wherein the germ cells comprise at least one inactivated endogenous immunoglobulin light chain locus in which the C.kappa. gene is deleted to prevent rearrangement of the locus and to prevent formation of a transcript of a rearranged locus and the expression of an endogenous immunoglobulin light chain from the inactivated locus, said method comprising the steps of:

• (a) deleting the C.kappa. gene from at least one endogenous light chain locus in a mouse embryonic stem cell to prevent rearrangement of said locus and to prevent formation of a transcript of a rearranged immunoglobulin light chain locus, the deletion being effected by a targeting vector comprising a gene encoding a selectable marker;
• and
• (b) producing from the embryonic stem cell a transgenic mouse whose germ cells comprise at least one immunoglobulin light chain locus in which the C.kappa. gene is deleted.

A method for producing a transgenic mouse and its progeny, wherein the somatic and germ cells comprise at least one inactivated endogenous immunoglobulin light chain locus in which the C.kappa. gene is deleted to prevent rearrangement of the locus and to prevent formation of a transcript of a rearranged locus and the expression of an endogenous immunoglobulin light chain from the inactivated locus, said method comprising the steps of:

• (a) deleting the C.kappa. gene from at least one endogenous light chain locus in a mouse embryonic stem cell to prevent rearrangement of the locus and to prevent formation of a transcript of a rearranged immunoglobulin light chain locus, the deletion being effected by a targeting vector comprising a gene encoding a selectable marker;
• (b) producing from the embryonic stem cell a transgenic mouse whose germ cells comprise at least one immunoglobulin light chain locus in which the C.kappa. gene is deleted, and
• (c) breeding the transgenic mouse as needed to produce a transgenic mouse and its progeny whose somatic and germ cells comprise at least one inactivated endogenous immunoglobulin light chain locus in which the C.kappa. gene is deleted.

A mouse embryonic stem cell with at least one inactivated endogenous immunoglobulin light chain locus in which the C.kappa. gene is deleted to prevent rearrangement of the locus and to prevent formation of a transcript of a rearranged immunoglobulin light chain locus, said inactivated endogenous locus comprising a gene encoding a selectable marker.

A transgenic mouse wherein the germ cells comprise at least one inactivated endogenous immunoglobulin light chain locus in which the C.kappa. gene is deleted to prevent rearrangement and to prevent formation of a transcript of a rearranged locus and the expression of an endogenous immunoglobulin light chain from the inactivated locus.

A transgenic mouse, wherein the somatic and germ cells comprise at least one inactivated endogenous immunoglobulin light chain locus in which the C.kappa. gene is deleted to prevent rearrangement and to prevent formation of a transcript of a rearranged locus and the expression of an endogenous immunoglobulin light chain from the inactivated locus.

A transgenic mouse, wherein the somatic and germ cells comprise inactivated endogenous immunoglobulin loci in which the C.kappa. gene is deleted to prevent rearrangement and to prevent formation of a transcript of a rearranged locus, wherein the transgenic mouse and progeny lack expression of endogenous immunoglobulin light chains.