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US 6,162,909 AGranted
Deprotection of RNA
AssigneeRibozyme Pharmaceuticals, Inc.
InventorsLaurent Bellon, Christopher T. Workman
Life of this patent
Estimating the term…
Abstract
Method for one-pot deprotection of RNA molecules.
Claims19
A process for one pot deprotection of RNA synthesized on the surface of a well in a plate having a defined number of wells, comprising the steps of:
- a) contacting said RNA with a mixture of anhydrous alkylamine, trialkylamine and a polar organic reagent in a predetermined proportions, at room temperature for about between 30 and 100 min under conditions suitable for the removal of nucleic acid base and phosphate protecting groups from said RNA;
- and
- b) contacting said base and phosphate deprotected RNA with a anhydrous triethylamine.hydrogen fluoride at about between 50.degree. C.-70.degree. C. under conditions suitable for the removal of 2'-OH protecting group.
A process for one pot deprotection of RNA synthesized on the surface of a well in a plate having a defined number of wells, comprising the steps of:
- a) contacting said RNA with a mixture of anhydrous methylamine, triethylamine and dimethylsulfoxide in proportions of 10, 3 and 13, respectively, at room temperature for about 90 min under conditions suitable for the removal of nucleic acid base and phosphate protecting groups from said RNA;
- and
- b) contacting said base and phosphate deprotected RNA with a anhydrous triethylamine.hydrogen fluoride at about between 65.degree. C. under conditions suitable for the removal of 2'-OH protecting group.
Dependencies are inferred from the claim text.