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US 6,160,204 AGranted
Polyphenol oxidase cDNA
AssigneeCornell Research Foundation, Inc.
InventorJohn C. Steffens
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Abstract
The present invention describes the cloning and sequencing of plant Polyphenol Oxidase (PPO) cDNAs which has subsequently permitted their use to genetically transform plants to achieve a variety of desired phenotypes.
Claims51
An isolated nucleic acid molecule encoding a plant polyphenol oxidase.
A method of obtaining DNA encoding a plant polyphenol oxidase, said method comprising:
- selecting a DNA molecule encoding a plant polyphenol oxidase, said DNA molecule having a nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:5, SEQ ID NO:14, and SEQ ID NO:17;
- designing an oligonucleotide probe for a plant polyphenol oxidase based on the nucleotide sequence of the selected DNA molecule;
- probing a genomic or cDNA library of a plant with the oligonucleotide probe;
- and
- obtaining clones from said library that are recognized by said oligonucleotide probe, so as to obtain DNA encoding a plant polyphenol oxidase.
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