Method for secretory production of human growth hormone
AssigneeMitsui Chemicals, Inc.
InventorsMasaru Honjo, Naokazu Naitoh, Hiroshi Uchida, Daisuke Mochizuki, Kazuya Matsumoto
Life of this patent
Estimating the term…
Abstract
A DNA encoding 20K hGH is connected directly to a gene encoding Escherichia coli OppA protein secretion signal, or a modified form thereof, and a DNA encoding signal peptidase 1 to construct a recombinant plasmid, E. coli is transformed by said plasmid and cells of the resulting E. coli transformant strain are cultured for secretory production of the 20K hGH in the E. coli periplasm. This method enables efficient secretory production of 20K hGH and easy isolation and purification of 20K hGH from the periplasm fraction because the level of impure proteins in the E. coli periplasm is low.
Claims4
An isolated secretion signal which is different from E. coli or Salmonella OppA secretion signals but is more than 60% homologous to said E. Coli OppA secretion signal in its amino acid sequence, characterized in that when the secretion signal is expressed as a fusion protein to the 20,000 kd human growth hormone a human growth hormone having a molecular weight of about 20,000 can be secreted and produced using a microorganism having a recombinant DNA in which the 3' end of a DNA encoding said secretion signal is operatively linked to the 5' end of a DNA encoding the human growth hormone having a molecular weight of about 20,000.
Dependencies are inferred from the claim text.