Hepatitis GB virus recombinant proteins and uses thereof
AssigneeAbbott Laboratories
InventorsTami J. Pilot-Matias, Thomas P. Leary, John N. Simons, Robert J. Carrick, Teresa K. Surowy, Suresh M. Desai, George J. Dawson, Anthony Scott Muerhoff, Isa K. Mushahwar
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Abstract
Recombinantly produced hepatitis GB Virus (HGBV) amino acid sequences useful for a variety of diagnostic and therapeutic applications, kits for using the HGBV amino acid sequences and antibodies which specifically bind to HGBV. Also provided are methods for producing antibodies, polyclonal or monoclonal, from the HGBV recombinantly produced amino acid sequences.
Claims27
An assay kit for determining the presence of hepatitis GB virus (HGBV) E2 antigen or antibody in a test sample comprising a container containing a polypeptide possessing at least one HGBV epitope of an HGBV E2 antigen, wherein said kit detects only HGBV E2 antigen or antibody present in said test sample and no other HGV antigens or antibodies in said test sample.
A method for detecting hepatitis GB virus (HGBV) E2 antibodies in a test sample suspected of containing said E2 antibodies, comprising:
- (a) contacting said test sample with a polypeptide of HGBV E2 antigen, for a time and under conditions sufficient to allow antigen/antibody complexes to form;
- and
- (b) detecting said complexes which contain the polypeptide, wherein said method detects only HGBV E2 antibodies in said test sample and no other HGV antibodies in said test sample.
A method for determining the clearance of hepatitis GB virus (HGBV) E2 antigen from a patient infected with HGBV, comprising:
- (a) contacting a test sample obtained from said patient with a HGBV E2 polypeptide, for a time and under conditions sufficient to allow antigen/antibody complexes to form;
- and
- (b) detecting said complexes, wherein the presence of said complexes is an indication of the clearance of said HGBV E2 antigen from said patient.
Plasmid pCHO/E2-336, having A.T.C.C. deposit No. CRL-12111.
Plasmid pSFV-ssE2-336, having A.T.C.C. deposit No. 98070.
Plasmid pCHO/E2-315, having A.T.C.C. deposit No. CRL-12110.
Plasmid pAcGP67A-E2C, having A.T.C.C. deposit No. 98072.
Dependencies are inferred from the claim text.