Separation columns and methods for manufacturing the improved separation columns

A chromatography device having a longitudinal axis of bulk liquid flow, the chromatography device comprising:

• (a) a substrate;
• (b) a plurality of collocated monolith support structures, each having a first end, a second end, and a wall therebetween,
• wherein
• the first end of each collocated monolith support structure is congruent with or in contact with the substrate, and
• the plurality of collocated monolith support structures are dimensioned and oriented in a uniform two-dimensional array defining a plurality of interconnected channels bound by the walls of the collocated monolith support structures and a surface of the substrate,
• wherein the plurality of interconnected channels are non-contiguous across two adjacent collocated monolith support structures in a direction perpendicular to the longitudinal axis of the chromatography device;
• and
• (c) a channel network, the channel network comprising a plurality of monolith structures, each monolith structure having a first end, a second end, and a wall therebetween,
• wherein
• the first end of each monolith structure is congruent with or in contact with the substrate,
• the plurality of monolith structures are dimensioned and oriented to define a second set of interconnected channels which are in fluid communication with the uniform two-dimensional array of interconnected channels, and
• the number of interconnected channels in the second set of interconnected channels counted in a direction perpendicular to the longitudinal axis of the chromatography device comprises less than the number of interconnected channels in the uniform two-dimensional array of interconnected channels by a factor of X, where X is the number of adjacent interconnected channels of the uniform two-dimensional array of interconnected channels which combine into a single channel within the second set of interconnected channels.

A chromatography device having a longitudinal axis of bulk liquid flow, the chromatography device comprising:

• (a) a substrate;
• (b) a plurality of collocated monolith support structures, each having a first end, a second end, and a wall therebetween,
• wherein
• the first end of each collocated monolith support structure is congruent with or in contact with the substrate,
• the plurality of collocated monolith support structures are dimensioned and oriented in a uniform two-dimensional array defining a plurality of interconnected channels bound by the walls of the collocated monolith support structures and a surface of the substrate,
• wherein the plurality of interconnected channels are non-contiguous across two adjacent collocated monolith support structures in a direction perpendicular to the longitudinal axis of the chromatography device;
• and
• (c) a cover plate associated with the second ends of the collocated monolith support structures,
• wherein
• the cover plate has a surface in fluid communication with the plurality of interconnected channels, and
• at least the walls of the collocated monolith support structures, the surface of the substrate, and the surface of the cover plate comprise a coating for chromatographically interacting with an analyte.