Microorganisms and plasmids for 2,4-dichlorophenoxyacetic acid (2,4-D) monooxygenase formation and process for the production of these plasmids and strains
AssigneeHoechst Schering AgrEvo GnbH
InventorsWolfgang R. Streber, Kenneth N. Timmis, Meinhart H. Zenk
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Abstract
The disclosure relates to the production and the use, by genetic engineering, of plasmids and bacterial strains containing, on a short, precisely characterizable DNA segment, the gene tfda or a gene almost identical to tfdA. The novel plasmids and microorganisms are suitable for the production of 2,4-D (2,4-dichlorophenoxyacetic acid)-monooxygenase, and as starting materials for the transfer, by genetic engineering of the 2,4-D-degrading property of this enzyme to various organisms.
Claims16
A recombinant gene, comprising
- a DNA sequence encoding a polypeptide having the biological activity of 2,4-D monooxygenase which is capable of being expressed in a plant, operably linked to
- a heterologous promoter capable of promoting the expression in a plant of a structural gene operably linked thereto.
A recombinant gene of claim 7, wherein the DNA sequence is
- the structural gene sequence of FIG. 10, except that the initiation codon is ATG,
- a DNA sequence differing therefrom by codon degeneracy, or
- a DNA sequence hybridizable therewith.
Dependencies are inferred from the claim text.