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US 6,153,375 AGranted
Method of making a library of compounds using a functionalized polymer support resin affixed to a laminar material
AssigneeCambridge Combinatorial Limited
InventorsRyszard Jurek Kobylecki, John Mark Francis Gardner
Life of this patent
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Abstract
The instant invention is directed to a method of making a library of compounds using a segmentable support material comprised of a particulate resin affixed to a porous laminar material.
Claims19
A method of making a library of compounds comprising the steps of:
- (a) individually identifying a plurality of discrete reaction zones on a laminar solid support material comprising a layer of a particulate, functionalized solid support resin affixed to a porous, inert, laminar material;
- (b) charging each of said reaction zones with a starting material;
- (c) sub-dividing the material to define at least two initial batches of reaction zones, wherein said batches are separated from one another;
- (d) applying at least two different reagents, one to each of the reaction zones in each initial batch, and recording the identity of those reaction zones to which each of said different reaction reagents is applied;
- (e) subjecting all reaction zones to reaction conditions which promote reaction to completion;
- (f) further sub-dividing each of said at least two initial batches into at least two alternative batches wherein said at least two alternative batches are separated from one another;
- (g) applying at least two different reagents, one to each of the reaction zones in each alternative batch, and recording the identity of those reaction zones to which each of said different reagents is applied;
- (h) subjecting all reaction zones to reaction conditions which promote reaction to completion;
- and
- (i) repeating steps (f) to (h) inclusive from zero to n times, as desired, wherein n is a whole number integer.
7 more elements
A method of making a library of compounds comprising the steps of:
- (a) providing a plurality of sheets of a solid support material to which compounds may be releasably bound, each such sheet bearing indicia identifying a plurality of individual reaction zones arranged in rows and columns in a two-dimensional array, said solid support material comprising a layer of a particulate, functionalized solid support resin affixed to a porous, inert, laminar material;
- (b) treating each sheet with respective first reagents to bind respective first constituents to each of the individual reaction zones on the sheets;
- (c) superposing the sheets resulting from step (b) to form a block and repeatedly subdividing the block parallel to one of its faces to form a plurality of stacks of strips derived from respective divided sheets, each strip bearing one of said rows of reaction zones;
- (d) treating each of the stacks of strips resulting from step (c) with respective second reagents to bind respective second constituents to the first constituents on the strips to form growing compounds;
- (e) reassembling the treated stacks of strips resulting from step (d) to reform the block and dividing the reformed block parallel to a second face thereof superposed at right angles to said first face so that each strip is divided into stacks of individual reaction zones;
- and
- (f) treating each of the stacks of individual reaction zones resulting from step (e) with respective third reagents to bind respective third constituents to the growing compounds already bound on the reaction zones to form stacks of treated individual reaction zones.
4 more elements
Dependencies are inferred from the claim text.