# US 6,152,194 A · Method and an equipment for portioning liquid consignments > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 6,152,194 A - Document ID: US6152194A - Status: Granted - Kind: A - Application number: 09/284,177 - Filed: 1999-04-08 - Granted: 2000-11-28 - Earliest claimed: 1996-10-08 - Assignees: Fluilogic Systems Oy - Inventors: Tuomas Tenkanen; Pekka Ronka - Web page: https://patentgrep.com/patent/US6152194A ## CPC - B01L 3/021: {Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids} - G01F 11/08: of the diaphragm or bellows type - G01F 11/086: {using an auxiliary pressure to cooperate with the diaphragm or bellows} - G01N 2035/1023: {using a valve in the tip or nozzle} - B01L: CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE - G01F: MEASURING VOLUME, VOLUME FLOW, MASS FLOW OR LIQUID LEVEL; METERING BY VOLUME - G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q) ## Abstract This invention relates to a method and an equipment for portioning of liquid consignments of specific amounts and to the packaging of these small liquid consignments in mass production. The portioning is executed with a bellows, which defines an air space of varying volume, that the air stream caused by the bellows establishes the injection and suction of a liquid. According to the invention the air space is connected to the suction tip (4), which holds a liquid space confined by a filter permeable to air stream but impermeable to liquid. When in the suction phase the liquid is sucked into the suction tip (4) by the motion of the liquid, and in the injection phase the reverse motion of the bellows is used in injecting the liquid consignment from the suction tip, and the injection of the liquid consignment of certain volume is based on a corresponding volume change in the air space of the bellows established by an equipment controlling the bellows. Serial portioning may take place so that a liquid volume of multiple times of the liquid consignments is sucked into the suction tip (4), after which the liquid consignments are portioned by the reversible motions of the bellows produced by the equipment controlling the bellows, which may be a step motor, for example. The suction tip may be removable, in which case the liquid to be portioned may be changed without having to clean the equipment, since the filter (17) in the suction tip prevents the contamination of the air space of the bellows. ## Claims ### Claim 1 A method for producing portions of liquid (20) by use of a suction tip (4) containing a liquid space (18) confined by a filter (17) permeable to an air stream but impermeable to said liquid and a bellows (1) defining an air space (2) connected through an air channel (3) to said suction tip, the method comprising drawing liquid to said suction tip by means of a movement of the bellows, and injecting said liquid from the suction tip by means of reversed movement of the bellows, characterized in that a plurality of liquid portions (20) of a specified size are produced in a series operation which comprises the steps of drawing an amount of liquid to the suction tip (4), said amount being a multiple of an individual portion (20) to be produced, and then injecting said portions one by one at a number of subsequent injection steps, by use of an actuator (6) producing sequential movements of the bellows each with a change of the volume of said air space (2) corresponding to the size of the liquid portion that is injected and wherein the suction tip (4) is cleaned by letting the bellows (1) blow air through it immediately before the suction of the liquid to the suction tip (4). ### Claim 2 (depends on 1) A method in accordance with claim 1, characterised in that the suction tip (4) is removable and holds a filter (17), which is a sterile aerosol filter, which is permeable to air stream but holds liquid drops. ### Claim 3 (depends on 2) A method in accordance with claim 2, characterized in that the actuator is a stepper motor (6) that is producing reversible movements of the bellows (1). ### Claim 4 (depends on 3) A method in accordance with claim 3, characterised in that the suction tip (4) is cleaned by letting the bellows (1) blow air through it immediately before the suction of the liquid to the suction tip. ### Claim 5 (depends on 4) A method in accordance with claim 4, characterised in that the method is used in portioning different liquids by changing the removable suction tip (4) in between portionings. ### Claim 6 (depends on 5) A method in accordance with claim 5, characterised in that the method is used in portioning of a liquid, such as an enzyme product, into packages (21). ### Claim 7 (depends on 6) A method in accordance with the claim 6, characterised in that the liquid volume sucked into the suction tip is approximately 500-2000 microliters. ### Claim 8 (depends on 6) A method in accordance with claim 6, characterised in that the quantity of liquid (20) to be portioned per package (21) is of the order of 5-500 microliters, preferably approximately 20-100 microliters. ### Claim 9 (depends on 1) A method in accordance with claim 1, characterized in that the actuator is a stepper motor (6) that is producing reversible movements of the bellows (1). ### Claim 10 (depends on 1) A method in accordance with claim 1, characterised in that the method is used in portioning different liquids by changing the removable suction tip (4) in between portionings. ### Claim 11 (depends on 1) A method in accordance with claim 1, characterised in that the method is used in portioning of a liquid enzyme product into packages (21). ### Claim 12 An equipment for producing portions of liquid (20) comprising a suction tip (4) containing a liquid space (18) confined by a filter (17) permeable to an air stream but impermeable to liquid, and a bellows (1) defining an air space (2) connected through an air channel (3) to said suction tip, to let liquid be drawn to the suction tip and injected from the same by means of reversible movements of the bellows, characterized in that the bellows (1) is connected to an actuator (6) adapted for production of a series of movements of the bellows so as to sequentially diminish the volume of said air space (2) and cause a series of liquid portions (20) to be injected one by one from the suction tip (4) wherein the suction tip (4) is structured to be cleaned using the bellows (1) to blow air through the suction tip (4) immediately before the suction of the liquid to the suction tip (4). ### Claim 13 (depends on 12) An equipment in accordance with the claim 12, characterised in that the suction tip (4) is removable and it comprises a filter (17), which is a sterile aerosol filter, which is permeable to air stream but impermeable to liquid drops. ### Claim 14 (depends on 13) An equipment in accordance with the claim 13, characterised in that the interior (2) of the bellows (1) is connected to the removable suction tip (4) via the air channel (3). ### Claim 15 (depends on 14) An equipment in accordance with claim 14, characterised in that the equipment controlling the bellows (1) is a stepper motor (6), the rotatory motion of an axle (7) used by the stepper motor being converted to a linear motion of the bellows (1). ### Claim 16 (depends on 15) An equipment in accordance with the claim 15, characterised in that the means for converting the motion is a pullable string or band woundable around the axle (7) of the motor (6). ### Claim 17 (depends on 12) The use of an equipment in accordance with the claim 12 in portioning of small quantities of liquid (20) into packages (21) in series production. ### Claim 18 (depends on 12) An equipment in accordance with claim 12, characterised in that the equipment controlling the bellows (1) is a stepper motor (6), the rotatory motion of an axle (7) used by the stepper motor being converted to a linear motion of the bellows (1).