Grafts made from amniotic membrane; methods of separating, preserving, and using such grafts in surgeries
OwnerScheffer C. G. Tsenginventor
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Abstract
A method for making, storing and using a surgical graft from human amniotic membrane; the resulting graft; and the storage solution. The amniotic membrane is obtained from human placenta, from which the chorion has been separated. Sheets of the amniotic membrane are cut to size and mounted on filter paper. The cells of the amniotic membrane are killed, preferably while being frozen and thawed in the storage solution. The storage solution comprises a culture medium and a hyperosmotic agent, wherein the hydration of the amniotic membrane is maintained. The membrane can be impregnated with therapeutic agents, prior to storage, for use in post surgical healing or other therapies.
Claims12
A method for obtaining human amniotic membrane and preparing it to be used as a surgical graft, comprising the steps of:
- obtaining human placenta and cleaning it;
- separating said placenta's amniotic membrane from its chorion, said amniotic membrane having cells and an extracellular matrix;
- mounting said amniotic membrane onto a substrate, with the epithelial surface of said membrane facing away from said substrate;
- and
- killing the cells of said membrane prior to using said membrane as a surgical graft and maintaining the integrity of said extracellular matrix.
Dependencies are inferred from the claim text.