Tat-SF: cofactor for stimulation of transcriptional elongation by HIV-1 Tat
AssigneeMassachusetts Institute of Technology
InventorsPhillip A. Sharp, Qiang Zhou
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Abstract
The invention pertains to a novel transcriptional activity factor, Tat-Stimulatory Factor, as well as genes encoding this factor and fragments and biologically functional variants thereof. The Tat-Stimulatory Factor is involved in the regulation of transcriptional elongation of HIV-1 by Tat. The invention also pertains to therapeutics involving the foregoing proteins and genes, and agents that bind to the foregoing proteins and genes. The invention also relates to methods of screening for a compound which binds to Tat-SF1, Tat-SF1-associated kinase and/or a complex of Tat-SF1 and Tat-SF1-associated kinase, as well as methods of screening for compounds which modulate Tat-SF1-mediated transcriptional activation,
Claims13
An isolated nucleic acid molecule encoding a Tat-Stimulatory Factor protein selected from the group consisting of
- (a) nucleic acid molecules which hybridize under stringent conditions to a molecule consisting of tie coding region of the nucleic acid sequence of SEQ ID NO:1 and which codes for the Tat-Stimulatory Factor protein,
- (b) nucleic acid molecules that differ from the nucleic acid molecules of (a) in codon sequence due to the degeneracy of the genetic code, and
- (c) complements of (a) and (b).
An isolated nucleic acid molecule selected from the group consisting of (a) a unique fragment of SEQ ID NO:1 between 12 and 2650 nucleotides in length, and (b) complements of (a).
Dependencies are inferred from the claim text.