Method for producing a purified hemoglobin product

A method for producing a purified hemoglobin product, comprising the steps of:

• a) loading a hemoglobin solution onto an anion exchange chromatography column;
• b) injecting to the column at least eleven column void volumes of an equilibrating tris(hydroxymethyl) aminomethane acetate buffer solution having a pH in a range of between about 8.2 and about 8.6;
• and
• c) injecting into the column a tris(hydroxymethyl) aminomethane acetate buffer solution having a pH lower than that of the equilibrating buffer solution, whereby a purified hemoglobin product elutes from the column.

A method for producing a purified hemoglobin product, comprising the steps of:

• a) loading a hemoglobin solution onto an anion exchange chromatography column, said loaded column initially being equilibrated to a pH greater than about 8.7;
• b) injecting into the column at least eleven column void volumes of an equilibrating buffer solution of tris(hydroxymethane) aminomethane acetate having a pH in a range of between about 8.2 and about 8.6;
• and
• c) injecting at least one tris(hydroxymethane) aminomethane acetate buffer solution into the column, said buffer solution having a pH lower than about 8.2, whereby a purified hemoglobin product elutes from the column.