Modified hemoglobin-like compounds and methods of purifying same
AssigneeBaxter Biotech Technology Sarl
InventorsStephen P. Trimble, Antony J. Mathews, Bruce A. Kerwin, David A. Marquardt, Spencer Anthony-Cahill, Janet K. Epp, Dominic G. Madril, David C. Anderson
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Abstract
The present invention relates to modified hemoglobin-like polypeptides containing multiple dialpha (or dibeta) domains. The present invention also relates to multimeric hemoglobin-like proteins comprising covalently joined hemoglobin-like moieties. Another aspect of the inention is directed at a purification method of hemoglobin-like polypeptides utilizing ion exchange chromatography.
Claims10
A globin-like polypeptide comprising two dialpha domains.
A multimeric hemoglobin-like protein comprising a core hemoglobin-like moiety to which each of at least two other hemoglobin-like moieties are directly attached to the core hemoglobin-like moiety.
A method for separation of molecular weight fractions of polymerized hemoglobin-like molecules to obtain substantially monodisperse hemoglobin solutions comprising:
- (a) contacting a polydisperse mixture of polymerized hemoglobin-like molecules with an ion exchange matrix;
- (b) washing the ion exchange matrix with a first buffer;
- (c) eluting the ion exchange matrix with a second buffer which can be the same or different from said first buffer to obtain a substantially monodisperse hemoglobin-like solution.
Dependencies are inferred from the claim text.