Fibrin microbeads prepared from fibrinogen, thrombin and factor XIII
AssigneeHadasit Medical Research Services and Development Ltd.
InventorsGerard Marx, Raphael Gorodetsky
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Abstract
The present invention provides fibrin microbeads that are biologically active and comprise extensively cross-linked fibrin(ogen) without using glutaraldehyde, and a method for preparing the fibrin microbeads. The present invention also provides a composition comprising cells bound to the fibrin microbeads, and methods for culturing and separating cells using the fibrin microbeads of the present invention. Finally, the present invention provides methods for transplanting cells and engineering tissue using the fibrin microbeads of the present invention.
Claims16
A method for producing fibrin microbeads in the absence of glutaraldehyde as a cross linking agent, said method comprising the sequential steps of:
- (i) preparing an aqueous solution comprising fibrinogen, thrombin and Factor XIII;
- (ii) prior to the onset of coagulation, contacting said aqueous solution with an oil heated to a temperature of about 50-80.degree. C. to form an emulsion;
- (iii) mixing the emulsion at a temperature of about 50-80.degree. C. until fibrin microbeads comprising extensively cross-linked fibrin(ogen) are obtained;
- and (iv) isolating the fibrin microbeads.
Dependencies are inferred from the claim text.