Process for the purification of serum albumin
AssigneeDSM Patents and Trademarks
InventorsCornelis Jacobus Van Der Laken, Marcellinus Petrus Johannes Piet
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Abstract
Recombinantly produced serum albumin is purified in a series of steps, optionally by incubation with an anion-exchange adsorbent, followed by affinity chromatography employing a hydrophobic solid phase and using a water-soluble lipid anion as desorbens in the aqueous phase. The immobile phase comprises a carrier coupled to a 2-mercapto or 2-hydroxy alkanoic acid.
Claims9
A lipophilic immobile phase for affinity chromatography comprising
- (a) a carrier activated with an epoxy compound that forms a spacer, that has up to about 10 C, and is linked to the carrier;
- and
- (b) a ligand, immobilized to the carrier through coupling with the spacer, wherein the ligand comprises a 2-mercapto C4-C14 alkanoic acid or a 2-hydroxy C4-C14 alkanoic acid, which immobile ligand selectively binds 40-50 kD fragments having Domains I and II of the mature serum albumin compared to intact recombinant serum albumin that has Domains I, II and III.
A composition comprising isolated recombinantly produced serum albumin which is 99.9% to 100% pure monomeric, mature serum albumin, wherein the composition is free of 40-50 kD serum albumin fragments having Domains I and II of mature serum albumin.
Dependencies are inferred from the claim text.