Phosphorylated fusion proteins
AssigneePestka Biomedical Laboratories, Inc.
InventorSidney Pestka
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Abstract
Modified proteins, modified interferons .alpha.'s and .beta.'s, phosphorylated modified proteins and DNA sequences encoding the above, applications and uses thereof. Modified phosphorylated Hu-IFN-.alpha.-like proteins are provided which carry an identifiable label such as a radio-label. Corresponding phosphorylatable Hu-IFN-.alpha.-like proteins which contain a putative phosphorylation site. DNA sequences which encode a Hu-IFN-.alpha.-like protein and contain a sequence encoding a putative phosphorylatable site. Appropriate expression vectors are used to transform compatible host cells of various microorganisms, such as E. coli. Numerous uses for the phosphorylated proteins are disclosed.
Claims14
A fusion protein, encoded by a contiguous coding sequence of an mRNA, comprising a first polypeptide sequence of a mammalian protein which is not normally phosphorylated in vivo and which has a desired bioactivity, and a second polypeptide sequence comprising a phosphorylation recognition sequence for a kinase, wherein the fusion protein, when phosphorylated on the phosphorylation recognition sequence, retains the desired bioactivity.
Dependencies are inferred from the claim text.