Epoxide hydrolase complexes and methods therewith

A purification method for an epoxide hydrolase, comprising:

• immobilizing a compound having the structure ##STR39## wherein Z is oxygen or sulfur, W is carbon or sulfur, X and Y is each independently nitrogen, oxygen, or sulfur, and X can further be carbon, at least one of R.sub.1 -R.sub.4 is hydrogen, R.sub.2 is hydrogen when X is nitrogen but is not present when X is sulfur or oxygen, R.sub.4 is hydrogen when Y is nitrogen but is not present when Y is sulfur or oxygen, R.sub.1 and R.sub.3 is each independently C.sub.1-20 substituted or unsubstituted alkyl, cycloalkyl, aryl, acyl, or heterocyclic, to a water-insoluble support;
• and
• eluting an aqueous solution having an epoxide hydrolase therein through the support.

An affinity separations article, comprising:

• a water insoluble support defining an exposed surface and having an immobilized compound thereon, the immobilized compound being derived from a compound having the structure ##STR40## wherein X and Y is each independently nitrogen, oxygen, or sulfur, and X can further be carbon, at least one of R.sub.1 -R.sub.4 is hydrogen, R.sub.2 is hydrogen when X is nitrogen but is not present when X is sulfur or oxygen, R.sub.4 is hydrogen when Y is nitrogen but is not present when Y is sulfur or oxygen, R.sub.1 and R.sub.3 is each independently C.sub.1-20 substituted or unsubstituted alkyl, cycloalkyl, aryl, acyl, or heterocyclic, the compound being derivatized for immobilization through one of R.sub.1 or R.sub.3, the immobilized compound capable of forming a complex with an epoxide hydrolase.