US 6,150,404 AGranted

Method to diagnose metabolic pyrethroid insecticide resistance

AssigneeBoard of Supervisors of Louisiana State University and Agricultural and Mechanical College

InventorsJames A. Ottea, Guomin Shan

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Abstract

The phenoxybenzyl moiety of conventional pyrethroids is a major site of oxidative metabolism in resistant tobacco budworms, Heliothis virescens (F.). This group was replaced with several P450 monooxygenase-inhibiting or oxidatively blocked groups. Several isomers were tested as insecticides or synergists for insecticides against tobacco budworms that were insecticide-susceptible or that expressed metabolic resistance to cypermethrin. Several compounds with insecticidal and synergistic activities were found. Activity was dependent on both geometric and stereochemical configurations. These compounds may be used in diagnosing the mechanism of resistance in field strains with acquired resistance to pyrethroids. Knowledge of the mechanism underlying acquired insecticide resistance helps farmers to control emerging resistant strains.

Claims11

Dependent claims

In a population of dipteran, homopteran, lepidopteran, blattarian, hymenopteran, hemipteran, isopteran, or coleopteran insects exhibiting resistance to a pyrethroid insecticide;

a method of diagnosing whether the pyrethroid resistance results from a metabolically-based resistance mechanism;
said method comprising exposing pyrethroid-resistant insects from the population to an effective amount of a compound selected from the group consisting of trans, 1R-fenfluthrin;
cis, 1R-fenfluthrin;
and cis, 1R-methylenedioxyphenyl-3-(2,2-dihalovinyl)-2,2-dimethylcyclopropanecarbox ylate;
and observing the fraction of exposed insects that are killed by said exposing step;
whereby the results of said diagnostic method indicate that a metabolic mechanism is primarily responsible for the resistance if at least a discriminatory percentage of the insects is killed by said exposing step;
and that a mechanism other than a metabolic mechanism, either alone or in conjunction with a metabolic mechanism, is responsible for the resistance if less than a discriminatory percentage of the insects is killed by said exposing step.

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Dependencies are inferred from the claim text.