BMP products
AssigneeGenetics Institute, Inc.
InventorsElizabeth A. Wang, John M. Wozney, Vicki A. Rosen
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Abstract
Purified BMP-2 and BMP-4 proteins and processes for producing them are disclosed. The proteins may be used in the treatment of bone and cartilage defects and in wound healing and related tissue repair.
Claims14
A method for induction selected from the group consisting of the induction of bone formation, cartilage formation and the formation of the combination thereof in a patient comprising administering an amount of a purified BMP-2 protein effective for inducing said formation of bone, cartilage and the combination thereof said BMP-2 protein produced by the steps of:
- (a) culturing under suitable conditions a cell transformed with a DNA sequence selected from the group consisting of
- (i) a cDNA comprising the nucleotide sequence from nucleotide #356 to #1543 of SEQ ID NO:3 encoding a subunit of BMP-2;
- and
- (ii) DNA sequences comprising a nucleotide sequence which encodes a subunit having the 98 amino acid sequence from amino acid #17 to amino acid #114 of SEQ ID NO:
- 4
- said DNA sequence in operative association with an expression control sequence therefor
- (b) recovering and purifying from said culture medium a protein wherein each subunit comprises the 98 amino acid sequence from amino acid #17 to amino acid #114 of SEQ ID NO:
- 4 said protein having induction activity selected from the group consisting of bone, cartilage and the combination thereof.
A method for induction selected from the group consisting of the induction of bone formation, cartilage formation and the formation of the combination thereof in a patient comprising administering an amount of a purified BMP-4 protein effective for inducing said formation of bone, cartilage and the combination thereof said BMP-4 protein produced by the steps of
- (a) culturing under suitable conditions a cell transformed with a DNA sequence selected from the group consisting of
- (i) a cDNA comprising the nucleotide sequence from nucleotide #403 to #1626 of SEQ ID NO:
- 5 encoding a subunit of BMP-4;
- and
- (ii) DNA sequences comprising a nucleotide sequence which encodes a subunit having the amino acid sequence from amino acid #19 to #116 of SEQ ID NO:6
- said DNA sequence in operative association with an expression control sequence therefor
- (b) recovering and purifying from said culture medium a protein wherein each subunit comprises the 98 amino acid sequence from amino acid #19 to amino acid #116 of SEQ ID NO:
- 6 said protein having induction activity selected from the group consisting of bone, cartilage and the combination thereof.
Dependencies are inferred from the claim text.