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AssigneeUniversite Pierre et Marie Curie
InventorsAvraham Halbreich, Domagoj Sabolovic, Claude Sestier, Danielle Geldwerth, Jean-Noel Pons, Jacky Roger
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The invention relates to a process for producing ferrofluid-forming magnetic particles substituted by DMSA (FFSH) that can be covalently coupled, directly or through a difunctional reactant, to an effector, which process comprises combining DMSA with ferrofluid-forming magnetic particles to form FFSS particles having disulfide bonds, and reducing the disulfide bonds of the FFSS at basic pH to form FFSH. The invention includes a composition comprising such FFSH magnetic particles. The invention also relates to a method in which FFSH magnetic particles are incubated with an effector comprising at least one functional group which forms an S--S, C--S, C--C, or C--N bond with DMSA, so that a covalent effector-FFSH complex is formed. The invention further includes a method for separating and/or distinguishing between molecules or cells having a ligand that forms an affinity complex with an effector-FFSH particle of the invention, and those molecules or cells that do not have such a ligand.
A method for obtaining ferrofluid-forming magnetic particles substituted with dimercaptosuccinic acid (DMSA) which are capable of being covalently coupled to an effector, the method comprising combining DMSA with ferrofluid-forming magnetic particles, thereby producing ferrofluid-forming particles having disulfide bonds (FFSS), and reducing the disulfide bonds of the FFSS at a basic pH to form ferrofluid-forming magnetic particles substituted with DMSA having reduced thiol groups (FFSH).
A composition comprising a ferrofluid-forming magnetic particle complexed with DMSA having reduced thiol groups FFSH.
A method for identifying molecules or cells having a ligand that forms an affinity complex with an effector coupled to FFSH, the method comprising:
Dependencies are inferred from the claim text.