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US 6,150,180 AGranted
High throughput screening assay systems in microscale fluidic devices
AssigneeCaliper Technologies Corp.
InventorsJohn Wallace Parce, Anne R. Kopf-Sill, Luc J. Bousse
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Abstract
The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems.
Claims14
A method of screening a plurality of test compounds for an effect on a biochemical system, comprising:
- providing a microfluidic device that comprises:
- a substrate having a plurality of discrete reaction channels disposed therein, each reaction channel comprising a detection window;
- a plurality of sources of a first component of a biochemical system disposed in the substrate, each source of first component being fluidly connected to a different one of the plurality of discrete reaction channels;
- and
- a plurality of sources of a second component of a biochemical system disposed in the substrate, each of the sources of second component being fluidly connected to a separate one of the plurality of discrete reaction channels;
- transporting the first and second components of the biochemical system into the reaction channel;
- introducing a different one of the plurality of test compounds into each of the plurality of discrete reaction channels, wherein the test compound contacts the first and second components;
- and
- detecting an effect, if any of each of the different test compounds on the interaction of the first and second components at the detection windows in each of the plurality of discrete reaction channels.
6 more elements
Dependencies are inferred from the claim text.