Method for introducing and expressing genes in animal cells, and live invasive bacterial vectors for use in the same
AssigneeUniversity of Maryland at Baltimore
InventorsRobert J. Powell, George K. Lewis, David M. Hone
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Abstract
A method for introducing and expressing genes in animal cells is disclosed comprising infecting said animal cells with live invasive bacteria, wherein said bacteria contain a eukaryotic expression cassette encoding said gene. The gene may encode, e.g., a vaccine antigen, an therapeutic agent, an immunoregulatory agent or a anti-sense RNA or a catalytic RNA.
Claims15
A method for introducing and expressing a gene in animal cells comprising infecting said animal cells with live invasive bacteria, wherein said bacteria contain a eukaryotic expression cassette encoding said gene, wherein said gene is expressed at detectable levels, and wherein said animals cells are cultured in vitro.
Dependencies are inferred from the claim text.