Nucleic acid transporter systems and methods of use
AssigneeBaylor College of Medicine
InventorsSavio L. C. Woo, Louis C. Smith, Richard J. Cristiano, Stephen Gottchalk, Jim Sparrow
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Abstract
Nucleic acid transporter systems for delivery of nucleic acid to a cell. The nucleic acid transporter includes a binding complex. The binding complex contains a binding molecule which non-covalently binds to the nucleic acid and covalently links to a surface ligand, nuclear ligand and/or a lysis agent. These may be linked to the binding molecule by spacers.
Claims52
Nucleic acid transporter system for delivering nucleic acid into a cell, comprising:
- a first nucleic acid binding complex comprising a first binding molecule noncovalently bound to said nucleic acid and covalently linked to a surface ligand;
- a second nucleic acid binding complex comprising a second binding molecule noncovalently bound to said nucleic acid and covalently linked to a nuclear ligand;
- wherein said first and second nucleic acid binding complexes are noncovalently bound to said nucleic acid simultaneously.
Nucleic acid transporter system for delivering nucleic acid into a cell, comprising:
- a first nucleic acid binding complex comprising a first binding molecule noncovalently bound to said nucleic acid and covalently linked to a surface ligand;
- a second nucleic acid binding complex comprising a second binding molecule noncovalently bound to said nucleic acid and covalently linked to a lysis agent;
- wherein said first and second nucleic acid binding complexes are noncovalently bound to said nucleic acid simultaneously.
A DNA transporter system for inserting specific DNA into a cell, comprising, a plurality of a first DNA binding complex, said complex including a first binding molecule non-covalently binding to DNA, said first binding molecule covalently linked to a surface ligand, said ligand binding to a cell surface receptor;
- a plurality of a second DNA binding complex, said complex including a second binding molecule non-covalently binding to DNA, said second binding molecule is a cationic peptide and is covalently linked to a nuclear ligand, said nuclear ligand is a nuclear localization sequence that recognizes and transports the transporter system through a nuclear membrane;
- wherein the nuclear ligand is attached to the second binding molecule by a spacer, wherein said plurality of first and second DNA binding complexes simultaneously, non-covalently bind the specific DNA.
A DNA transporter for inserting specific DNA into a cell, comprising,
- a plurality of a first DNA binding complex, said complex including a first binding molecule non-covalently binding to DNA, said first binding molecule attached to a first spacer, said first spacer also attached to a surface ligand, said ligand binding to a cell surface receptor;
- a plurality of a second DNA binding complex, said complex including a second binding molecule non-covalently binding to DNA, said second binding molecule is a cationic peptide and is attached to a second spacer, said second spacer also attached to a nuclear ligand, said nuclear ligand is a nuclear localization sequence that recognizes and transports the transporter system through a nuclear membrane;
- a plurality of a third DNA binding complex, said complex including a third binding molecule non-covalently binding to DNA, said third binding molecule attached to a third spacer, said third spacer also attached to a virus;
- wherein said plurality of first, second and third DNA binding complexes simultaneously, non-covalently bind to the specific DNA.
Dependencies are inferred from the claim text.