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US 6,150,160 AGranted
Compositions and methods of use of mammalian retrotransposons
AssigneesThe John Hopkins University, The Trustees of the University of Pennsylvania
InventorsHaig H. Kazazian, Jr., Jef D. Boeke, John V. Moran, Beth A. Dombroski
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Abstract
The invention relates to an isolated DNAc molecule comprising a promoter P and an L1 cassette sequence comprising a core L1 retrotransposon element and methods of use thereof.
Claims3
An isolated DNAc molecule comprising a promoter P and an L1 cassette sequence;
- wherein said L1 cassette sequence comprises a core retrotransposon element;
- wherein said core L1 retrotransposon element comprises a 5' UTR, ORF1, ORF2 comprising EN and RT domains, a 3' UTR, a poly A signal, and a vector sequence comprising at least one origin of DNA replication and a DNA sequence encoding at least one selectable marker protein;
- wherein said isolated DNAc molecule further comprises a fragment of non-L1 DNA and a promoter P' for expression of the non-L1 DNA, wherein said non-L1 DNA and promoter P' are positioned within said 3' UTR or between said 3' UTR and said poly A signal.
Dependencies are inferred from the claim text.