US 6,150,134 A · Purified hepatitis C virus envelope proteins for diagnostic and therapeutic use

Abstract

The present invention relates to a method for purifying recombinant HCV single or specific oligomeric envelope proteins selected from the group consisting of E1 and/or E2 and/or E1/E2, characterized in that upon lysing the transformed host cells to isolate the recombination expressed protein a disulphide bond cleavage or reduction step is carried out with a disulphide bond cleavage agent. The present invention also relates to a composition isolated by such a method. The present invention also relates to the diagnostic and therapeutic application of these compositions. Furthermore, the invention relates to the use of HCV E1 protein and peptides for prognosing and monitoring the clinical effectiveness and/or clinical outcome of HCV treatment.

Claims26

Dependent claims

A recombinant HCV single or specifically oligomerized envelope viral protein selected from the group consisting of E1, E2 or E1/E2 viral proteins obtained from a method comprising the steps of

(a) optionally lysing host cells expressing said viral protein;
(b) optionally recovering said viral protein;
(c) cleaving disulphide bonds of said viral protein with a disulphide bond cleaving agent to form a cleaved protein;
(d) preventing disulphide bond reformation of said cleaved viral protein with at least one of an SH group blocking agent and low pH condition;
and
(e) purifying the cleaved viral protein obtained in step (d) to produce a viral protein which is at least 80% purified.

3 more elements

Dependencies are inferred from the claim text.

Purified hepatitis C virus envelope proteins for diagnostic and therapeutic use

Life of this patent

Purified hepatitis C virus envelope proteins for diagnostic and therapeutic use

Life of this patent

Abstract

Claims26