Back to results
US 6,150,123 AGranted
Affinity biotinylation
AssigneeCentre Integre de Recherches Biocliniques sur le Sida (CIRBS)
InventorAntonio Cosma
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method for biotinylating a desired subset of a larger population of proteins, wherein the subset of proteins is first selectively bound to a solid phase, thereby separating it from undesired proteins of the population, and then, still bound to solid phase, it is biotinylated. Unreacted biotin may then be washed from the solid phase, and the biotinylated protein may be uncoupled from the solid phase.
Claims12
A method for detectably labeling with biotin a subset of a larger population of proteins comprising the following steps:
- (i) contacting the larger population of proteins with an affinity element, attached to a solid phase element, which selectively binds to the subset of proteins, under conditions which permit binding of the affinity element to the subset of proteins to occur;
- (ii) removing protein not bound to the solid phase element;
- (iii) linking biotin to the subset of proteins bound to the solid phase element;
- and
- (iv) eluting the subset of proteins linked to biotin.
2 more elements
Dependencies are inferred from the claim text.