NF-AT polypeptides and polynucleotides

A method for identifying a compound which modulates NF-AT activity, comprising

• (i) contacting an isolated NF-AT polypeptide, or portion thereof sufficient for forming an NF-AT protein-DNA complex, with a nucleic acid including an NF-AT binding site and a test compound in conditions under which, but for the presence of the test compound, an NF-AT protein-DNA complex is formed, wherein the NF-AT polypeptide is encoded by a nucleic acid which hybridizes to a nucleic acid having SEQ ID NO:
• 45 in 5.times. SSC at 42.degree. C.;
• and
• (ii) determining the amount of NF-AT protein-DNA complex formed in the presence relative to the absence of the test compound,
• such that a difference in the amount of NF-AT protein-DNA complex formed in the presence relative to the absence of the test compound indicates that the test compound modulates NF-AT activity.

A method for identifying a compound which modulates NF-AT activity, comprising

• (i) contacting a cell comprising an NF-AT polypeptide or portion thereof and a reporter gene operably linked to a promoter comprising an NF-AT binding site with a test compound, wherein the NF-AT polypeptide is encoded by a nucleic acid which is heterologous with respect to the cell and which hybridizes to a nucleic acid having SEQ ID NO. 45 in 5.times. SSC at 42.degree. C.;
• and
• (ii) determining the level of expression of the reporter gene in cells that were contacted with the test compound relative to cells that were not contacted with the test compound,
• such that a difference in the level of expression of the reporter gene in cells contacted with the test compound relative to cells that were not contacted with the test compound indicates that the compound modulates NF-AT activity.

A method for identifying a compound which modulates phosphorylation of an NF-AT polypeptide, comprising

• (i) contacting an isolated NF-AT polypeptide or portion thereof that can be phosphorylated with a mixture and a compound in conditions under which, but for the compound, phosphorylation of NF-AT is modulated by the mixture, wherein the NF-AT polypeptide is encoded by a nucleic acid which hybridizes to a nucleic acid having SEQ ID NO:
• 45 in 5.times. SSC at 42.degree. C.;
• and
• (ii) determining the level of phosphorylation of the NF-AT polypeptide or portion thereof in the presence relative to the absence of the compound,
• such that a difference in the level of phosphorylation of the NF-AT polypeptide or portion thereof in the presence relative to the absence of the compound indicates that the compound modulates phosphorylation of NF-AT.

A method for identifying a compound which modulates phosphorylation of an NF-AT polypeptide, comprising

• (i) contacting a cell comprising an NF-AT polypeptide or portion thereof that can be phosphorylated with a compound, wherein the NF-AT polypeptide is encoded by a nucleic acid which hybridizes to a nucleic acid having SEQ ID NO:
• 45 in 5.times. SSC at 42.degree. C., and
• (ii) determining the level of phosphorylation of the NF-AT polypeptide or portion thereof in cells contacted with the compound, relative to cells that were not contacted with the compound,
• such that a difference in the level of phosphorylation of the NF-AT polypeptide or portion thereof in the cell contacted with the compound relative to the cell that was not contacted with the compound indicates that the compound modulates phosphorylation of NF-AT.

A method for identifying a compound which affects the cellular location of an NF-AT polypeptide, comprising

• (i) contacting a cell comprising an NF-AT polypeptide, or portion thereof sufficient for translocation from the cytoplasm to the nucleus, with a test compound, wherein the NF-AT polypeptide is encoded by a nucleic acid which hybridizes to a nucleic acid having SEQ ID NO:
• 45 in 5.times. SSC at 42.degree. C.;
• and
• (ii) determining the cellular location of the NF-AT polypeptide after step (i),
• such that a difference in the cellular location of the NF-AT polypeptide in the cell contacted with the test compound relative to a cell that was not contacted with the test compound indicates that the test compound affects the cellular location of NF-AT.

A method for identifying a compound which modulates the activity of an NF-AT polypeptide, comprising

• (i) contacting an isolated NF-AT polypeptide or portion thereof sufficient for interacting with a molecule, with the molecule and a compound in conditions under which, but for the presence of the compound, the NF-AT polypeptide or portion thereof and the molecule interact, wherein the NF-AT polypeptide comprises at least 25 amino acids having an amino acid sequence which is at least 80% identical to an amino acid sequence of SEQ ID NO:
• 38, wherein the percent identity is determined with the algorithm (GAP, BESTFIT, or FASTA in the Wisconsin Genetics Software Package Release 7.0, using default gap weights;
• and
• (ii) determining the level of interaction between the NF-AT polypeptide or portion thereof and the molecule in the presence relative to the absence of the compound,
• such that a difference in the level of interaction between the NF-AT polypeptide or portion thereof and the molecule in the presence relative to the absence of the compound indicates that the compound modulates the activity of an NF-AT polypeptide.

A method for identifying a compound which modulates NF-AT activity, comprising

• (i) contacting an isolated NF-AT polypeptide, or portion thereof sufficient for forming an NF-AT protein-DNA complex, with a nucleic acid including an NF-AT binding site and a test compound in conditions under which, but for the presence of the test compound, an NF-AT protein-DNA complex is formed, wherein the NF-AT polypeptide comprises at least 25 amino acids having an amino acid sequence which is at least 80% identical to an amino acid sequence of SEQ ID NO:
• 38, wherein the percent identity is determined with the algorithm GAP, BESTFIT, or FASTA in the Wisconsin Genetics Software Package Release 7.0, using default gap weights;
• and
• (ii) determining the amount of NF-AT protein-DNA complex formed in the presence relative to the absence of the test compound,
• such that a difference in the amount of NF-AT protein-DNA complex formed in the presence relative to the absence of the test compound indicates that the test compound modulates NF-AT activity.

A method for identifying a compound which modulates NF-AT activity, comprising

• (i) contacting a cell comprising an NF-AT polypeptide, which is heterologous with respect to the cell, or portion thereof and a reporter gene operably linked to a promoter comprising an NF-AT binding site with a test compound, wherein the NF-AT polypeptide comprises at least 25 amino acids having an amino acid sequence which is at least 80% identical to an amino acid sequence of SEQ ID NO:
• 38, wherein the percent identity is determined with the algorithm GAP, BESTFIT, or FASTA in the Wisconsin Genetics Software Package Release 7.0, using default gap weights;
• and
• (ii) determining the level of expression of the reporter gene in cells that were contacted with the test compound relative to cells that were not contacted with the test compound,
• such that a difference in the level of expression of the reporter gene in cells contacted with the test compound relative to cells that were not contacted with the test compound indicates that the compound modulates NF-AT activity.

A method for identifying a compound which modulates phosphorylation of an NF-AT polypeptide, comprising

• (i) contacting an isolated NF-AT polypeptide or portion thereof that can be phosphorylated with a mixture and a compound in conditions under which, but for the compound, phosphorylation of NF-AT is modulated by the mixture, wherein the NF-AT polypeptide comprises at least 25 amino acids having an amino acid sequence which is at least 80% identical to an amino acid sequence of SEQ ID NO:
• 38, wherein the percent identity is determined with the algorithm GAP, BESTFIT, or FASTA in the Wisconsin Genetics Software Package Release 7.0, using default gap weights;
• and
• (ii) determining the level of phosphorylation of the NF-AT polypeptide or portion thereof in the presence relative to the absence of the compound,
• such that a difference in the level of phosphorylation of the NF-AT polypeptide or portion thereof in the presence relative to the absence of the compound indicates that the compound modulates phosphorylation of NF-AT.

A method for identifying a compound which modulates phosphorylation of an NF-AT polypeptide, comprising

• (i) contacting a cell comprising an NF-AT polypeptide or portion thereof that can be phosphorylated with a compound, wherein the NF-AT polypeptide comprises at least 25 amino acids having an amino acid sequence which is at least 80% identical to an amino acid sequence of SEQ ID NO:
• 38, wherein the percent identity is determined with the algorithm GAP, BESTFIT, or FASTA in the Wisconsin Genetics Software Package Release 7.0, using default gap weights;
• and
• (ii) determining the level of phosphorylation of the NF-AT polypeptide or portion thereof in cells contacted with the compound, relative to cells that were not contacted with the compound,
• such that a difference in the level of phosphorylation of the NF-AT polypeptide or portion thereof in the cell contacted with the test compound relative to the cell that was not contacted with the test compound indicates that the compound modulates phosphorylation of NF-AT.

A method for identifying a compound which affects the cellular location of an NF-AT polypeptide, comprising

• (i) contacting a cell comprising an NF-AT polypeptide, or portion thereof sufficient for translocation from the cytoplasm to the nucleus, with a test compound, wherein the NF-AT polypeptide comprises at least 25 amino acids having an amino acid sequence which is at least 80% identical to an amino acid sequence of SEQ ID NO:
• 38, wherein the percent identity is determined with the algorithm GAP, BESTFIT, or FASTA in the Wisconsin Genetics Software Package Release 7.0, using default gap weights;
• and
• (ii) determining the cellular location of the NF-AT polypeptide after step (i),
• such that a difference in the cellular location of the NF-AT polypeptide in the cell contacted with the test compound relative to a cell that was not contacted with the test compound indicates that the test compound affects the cellular location of NF-AT.