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US 6,150,095 AGranted
Method for analyzing a polynucleotide containing a variable sequence
AssigneeOxford Gene Technology Limited
InventorsEdwin Mellor Southern, Clare Elizabeth Pritchard, Stephen Charles Case-Green
Life of this patent
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Abstract
A method of analyzing a polynucleotide target involves incubating the target with an oligonucleotide probe, generally an array of immobilised oligonucleotide probes, to form a duplex, and using ligase or polymerase to extend one chain of the duplex. A point mutation or variable number tandem repeat section may be analysed. Arrays of immobilised oligonucleotides are provided for use in the method.
Claims5
A method of analysis which comprises:
- providing a polynucleotoide target including a nucleotide at a specified position, and two or more different oligonucleotide probes tethered to different locations of a support in the form of an array, each said probe being complementary to the target including an expected variant of the target and terminating at or close to the said specified position;
- and
- performing the steps:
- a) incubating the target with the probes to form a duplex,
- b) incubating the duplex under ligation conditions with a labelled oligonucleotid ementary to the target,
- c) and monitoring ligation in step b) as an indication of a point mutation at the specified position in the target.
3 more elements
A method of analysis which comprises:
- providing a polynucleotide target having a variable number tandem repeat section and a flanking section, and two or more different oligonucleotide probes tethered to different locations of a support in the form of an array, each said probe having a section complementary to a repeat section and a flanking section of the target;
- and
- performing the steps:
- a) incubating the target with the probes to form a duplex,
- b) incubating the duplex with a labelled oligonucleotide and/or at least one labelled nucleotide under chain extension conditions,
- c) and monitoring chain extension as an indication of the length of the variable number repeat section of the target.
3 more elements
Dependencies are inferred from the claim text.