Use of an osmolyte for reducing or abolishing no-covalent interactions of biological molecules to inert surfaces
AssigneeQiagen GmbH
InventorsElmar Maier, Igor Ivanov
Life of this patent
Estimating the term…
Abstract
The present invention relates to the use of an osmolyte for reducing or abolishing non-covalent interactions of biological molecules to inert surfaces. Furthermore, the present invention relates to kits that may be employed for uses in accordance with the present invention.
Claims17
A method for reducing or abolishing non-covalent interactions between an inert surface and a biological molecule, comprising adding a zwitterionic osmolyte of the structural formula:
- ##STR2## wherein R1, R2, and R3 are each selected from the group consisting of H, CH.sub.3, C.sub.2 H.sub.5 or any other alkyl, and R' is any amino acid residue, prior to or simultaneously with adding the biological molecule.
A kit comprising
- (a) a concentrated stock solution of an osmolyte as specified in any of the preceeding claims;
- (b) a reaction buffer formulation containing an osmolyte as specified in any of the preceeding claims;
- and/or
- (c) an enzyme formulation containing an osmolyte as specified in any of the preceding claims.
A method for reducing or abolishing non-covalent interactions in a PCR reaction between an inert surface and a biological molecule, comprising adding a zwitterionic osmolyte of the structural formula:
- ##STR3## wherein R1, R2, and R3 are each selected from the group consisting of H, CH.sub.3, C.sub.2 H.sub.5 or any other alkyl, and R' is any amino acid residue,
- to the inert surface prior to or simultaneously with adding the biological molecule.
Dependencies are inferred from the claim text.