Prolonged storage of red blood cells and composition
AssigneeThe United States of America as represented by the Secretary of the Army
InventorsJohn R. Hess, Tibor J. Greenwalt
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Abstract
Novel additive solutions useful for the storage of human red blood cells (RBCs) under refrigerated conditions are disclosed. Also disclosed is a method of using the additive solutions in an appropriate volume to preserve RBCs at about 1 to 6.degree. C. for up to 10 weeks. Additive solutions and processes in accordance with the present invention allow the viable storage of human RBCs for an extended period of time in a solution which is directly infusible in humans.
Claims21
An additive solution for prolonged storage of red blood cells at 1 to 6.degree. C., said solution consisting essentially of adenine at 1 to 3 mM, dextrose at 20 to 115 mM, Na.sub.2 HPO.sub.4 at 4 to 15 mM, mannitol at 15 to 60 mM, and 20-130 mM of a physiologically acceptable sodium salt wherein the pH is 8.0 to 8.8 measured at room temperature.
A method of preserving red blood cells (RBCs) for an extended period of time comprising:
- (a) mixing a sample of whole blood containing the RBCs to be stored and plasma with an anticoagulant solution, forming thereby a suspension of whole blood;
- (b) treating the whole blood suspension to concentrate the RBCs from the plasma, forming thereby packed RBCs;
- (c) mixing the packed RBCs with an appropriate amount of an additive solution consisting essentially of adenine at 1 to 3 mM, dextrose at 20 to 115 mM, Na.sub.2 HPO.sub.4 at 4 to 15 mM, mannitol at 15 to 60 mM, and 20-130 mM of a physiologically acceptable sodium salt, said solution having an osmolarity of 200 to about 310 mOsm and a pH of 8 to about 8.8 measured at room temperature, thereby forming a suspension of RBCs;
- (d) cooling said suspension of RBCs to 1 to about 6.degree. C.;
- and
- (e) storing said cooled suspension of RBCs according to standard blood bank procedures for a period of 8 to about 10 weeks.
A directly infusible red blood cell storage suspension comprising red blood cells (RBCs) suspended in a solution comprising adenine at 1 to 3 mM, dextrose at 20 to 115 mM, Na.sub.2 HPO.sub.4 at 4 to 15 mM, mannitol at 15 to 60 mM, and 20-130 mM of a physiologically acceptable sodium salt chosen from the group consisting of:
- sodium chloride and sodium acetate.
An additive solution for prolonged storage of red blood cells at 1 to 6.degree. C., said solution consisting essentially of adenine at 2 mM, dextrose at 110 mM, Na.sub.2 HPO.sub.4 at 12 mM, mannitol at 55 mM, and 26 mM of a physiologically acceptable sodium salt wherein the pH is 8.0 to 8.8 measured at room temperature.
An additive solution for prolonged storage of red blood cells at 1 to 6.degree. C., said solution consisting essentially of adenine at 2 mM, dextrose at 50 mM, Na.sub.2 HPO.sub.4 at 9 mM, mannitol at 20 mM, and 75 mM of a physiologically acceptable sodium salt wherein the pH is 8.0 to 8.8 measured at room temperature.
Dependencies are inferred from the claim text.