Human fibroblast diffusable factors
AssigneeAlberta Cancer Board
InventorsRazmik Mirzayans, Malcolm C. Paterson
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Abstract
The present invention provides for numerous cell factors involved in a novel cellular pathway that is activated in response to ionizing radiation. Several cell factor activities are described which either complement the radioresistant DNA synthesis phenotype of Ataxia Telangiectasia cells, or inhibit DNA synthesis in the cell. Other cell factor activities are described which inhibit mitosis by arresting the cell cycle prior to cell division. It is contemplated that compositions comprising the subject factors will be useful as both research tools, and as therapeutic agents.
Claims35
A method of making a composition comprising at least about 1 unit/ml to about 16,000 units/ml of DNA synthesis inhibiting factor (SIF) activity or at least about 1 unit/ml mitosis inhibiting factor (MIF), comprising the steps of:
- a) irradiating, with from about one to about six Gy .gamma. rays, a culture of normal, fibroblast cells;
- b) incubating said cells for a period of about 24 hours;
- c) releasing said SIF or MIF from said cells;
- d) centrifuging said culture;
- and
- e) concentrating the supernatant from step (d).
A method of making a composition comprising at least about 1 unit/ml of MIF activity, comprising the steps of:
- a) irradiating, with about 40 Gy .gamma. rays, a culture of human tumor cells;
- b) incubating said cells for a period of about 0.5 hours;
- c) reasing said MIF from said cells;
- d) centrifuging said culture;
- and
- e) concentrating the supernatant from step d.
Dependencies are inferred from the claim text.