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AssigneeBiocine S.p.A.
InventorsMario Domenighini, Rino Rappuoli, Mariagrazia Pizza, Wim Hol
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An immunogenic detoxified protein comprising the amino acid sequence of subunit A of cholera toxin (CT-A) or subunit A of an Escherichia coli heat labile toxin (LT-A) or a fragment thereof wherein one or more amino acids at, or in positions corresponding to Val-53, Ser-63, Val-97, Tyr-104 or Pro-106 are replaced with another amino acid or deleted. Examples of specific replacements include Val-53-Asp, Val-53-Glu, Val-53-Tyr, Ser-63-Lys, Val-97-Lys, Val-97-Tyr, Tyr-104-Lys, Tyr-104-Asp, Tyr-104-Ser, Pro-106-Ser. The immunogenic detoxified protein is useful as vaccine for Vibrio cholerae or an enterotoxigenic strain of Escherichia coli and is produced by recombinant DNA means by site-directed mutagenesis.
An immunogenic detoxified protein comprising an amino acid sequence of subunit A of a cholera toxin or a fragment thereof or an amino acid sequence of subunit A of an Escherichia coli heat labile toxin or a fragment thereof comprising one or more of the following amino acid replacements at or in positions corresponding to amino acids in the sequence of subunit A of mature cholera toxin:
A vaccine comprising an amino acid sequence of subunit A of a cholera toxin or an amino acid sequence of subunit A of an Escherichia coli heat labile toxin comprising one or more of the following amino acid replacements at or in positions corresponding to amino acids in the sequence of subunit A of mature cholera toxin:
An immunogenic detoxified protein comprising an amino acid sequence of subunit A of a cholera toxin or a fragment thereof or an acid sequence of subunit A of an Escherichia coli heat labile toxin or a fragment thereof wherein the position corresponding to Ser-63 in the sequence of subunit A of mature cholera toxin is replaced with lysine, wherein said fragment comprises from about 3 to about 5 amino acids of at least one of the regions containing one of the amino acids to be replaced.
An immunogenic detoxified protein comprising an amino acid sequence of subunit A of a cholera toxin or a fragment thereof or an amino acid sequence of subunit A of an Escherichia coli heat labile toxin or a fragment thereof wherein the position corresponding to Pro-106 in the sequence of subunit A of mature cholera toxin is replaced with serine, wherein said fragment comprises from about 3 to about 5 amino acids of at least one of the regions containing one of the amino acids to be replaced.
Dependencies are inferred from the claim text.