Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
AssigneeXy, LLC
InventorsGeorge Seidel, Lisa Herickhoff, John Schenk
Life of this patent
Estimating the term…
Abstract
Improved flow cytometer system particularly adapted to use for sex-selected sperm sorting include enhanced sheath fluid and other strategies which minimize stress on the sperm cells, including a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial applications of sperms samples as well as the resulting animals may be achieved.
Claims32
A flow cytometer system for isolating desired cells comprising:
- a. a cell source which supplies cells to be analyzed by the flow cytometer;
- b. a sheath fluid source which creates a sheath fluid environment for said cells which contains about 2.9% sodium citrate;
- c. a nozzle through which said cells pass while subjected to said sheath fluid environment;
- d. an oscillator which acts upon said sheath fluid as it passes through said nozzle;
- e. a cell sensing system which responds to said cells;
- f. a sorter discrimination system which acts to sort cells having a desired characteristic;
- and
- g. a collector into which cells having a desired characteristic are placed.
A flow cytometer system for isolating desired cells comprising:
- a. a cell source which supplies cells to be analyzed by the flow cytometer;
- b. a sheath fluid source which creates a sheath fluid environment for said cells which contains a hepes buffered medium;
- c. a nozzle through which said cells pass while subjected to said sheath fluid environment;
- d. an oscillator which acts upon said sheath fluid as it passes through said nozzle;
- e. a cell sensing system which responds to said cells;
- f. a sorter discrimination system which acts to sort cells having a desired characteristic;
- and
- g. a collector into which cells having a desired characteristic are placed.
A flow cytometer system for isolating desired cells comprising:
- a. a cell source which supplies cells to be analyzed by the flow cytometer;
- b. a sheath fluid source which creates a sheath fluid environment for said cells;
- c. a nozzle through which said cells pass while subjected to said sheath fluid environment;
- d. an oscillator which acts upon said sheath fluid as it passes through said nozzle;
- e. a cell sensing system which responds to said cells;
- f. a sorter discrimination system which acts to sort cells having a desired characteristic;
- and
- g. a collector into which cells having a desired characteristic are placed and which comprises a citrate collector fluid containing about six percent egg yolk.
A flow cytometer system for isolating desired cells comprising:
- a. a cell source which supplies cells to be analyzed by the flow cytometer;
- b. a chemically coordinated sheath fluid source which creates a sheath fluid environment for said cells which is selected to be coordinated with both a pre-sort and a post-sort cell fluid environment;
- c. a nozzle through which said cells pass while subjected to said sheath fluid environment;
- d. an oscillator which acts upon said sheath fluid as it passes through said nozzle;
- e. a cell sensing system which responds to said cells;
- f. a sorter discrimination system which acts to sort cells having a desired characteristic;
- and
- g. a collector into which cells having a desired characteristic are placed.
Dependencies are inferred from the claim text.