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AssigneesShimadzu Corporation, Wako Pure Chemical Industries, Ltd.
InventorsKenji Nakamura, Shinji Satomura, Masayoshi Hayashi, Hideyoshi Arashima, Tetsushi Okuyama, Nobuhiro Hanafusa, Koji Tanimizu
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The object of the present invention is to provide a method for separating components in a liquid specimen and an apparatus used in said method, by which it is possible to avoid carryover even when the specimen injector is not washed using washing solution and also to avoid the increase of inner pressure in column of the separation device or ineffective separating operation even when many liquid specimens are separated by many times using the same separation device. In the present invention, the liquid specimen is introduced into the separation device from a direction opposite to eluting direction of the separation device which retains an adsorbent, and by separating and eluting the specimen from eluting outlet together with the eluant, trace quantity of insoluble substances and the specimen remained on the specimen introducing passage can be removed by elution procedure. As a result, it is possible to avoid carryover, to prevent the increase of inner pressure in the column of the separation device and to prevent ineffective separating operation caused by clogging of the column.
A method for separating two or more components in a liquid specimen, utilizing difference of adsorption to an adsorbent, comprising the steps of introducing the liquid specimen from a direction opposite to eluting direction of a separation device which retains the adsorbent and has an eluant inlet connected to an eluant supply, and of separating and eluting the components from the eluting outlet together with an eluant, whereby specimen carry over is avoided.
Dependencies are inferred from the claim text.