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AssigneeStichting Scheikundig Onderzoek in Nederland
InventorsJosephus Christianus Maria Smeekens, Michael Johannes Marcus Ebskamp, Hendrikus Andrianus Maria Geerts, Petrus Jacobus Weisbeek
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The invention relates to a method for producing oligosaccharides, comprising selecting a gene which codes for an enzyme which is capable of converting sucrose into an oligosaccharide; linking the gene to suitable transcription-initiation and transcription-termination signals in order to provide an expression construct; transforming a suitable plant cell with the expression construct; transforming a suitable plant cell with the expression construct; regenerating a transgenic plant from the transformed plant cell; culturing the transgenic plant under conditions enabling the expression and activity of the enzyme; and isolating the oligosaccharides from the transgenic plant. The invention further relates to the product obtained by means of the method and to the use thereof, in addition to transgenic plants and parts thereof which are capable of producing oligosaccharides.
A method for producing oligosaccharides having a low degree of polymerization, comprising the steps of:
A DNA-construct for expressing a bacterial fructosyltransferase enzyme which converts sucrose into an oligosaccharide having a low degree of polymerization in a plant or plant cell, comprising a gene which codes for the bacterial fructosyltransferase enzyme, wherein the gene is mutated so that the resultant oligosaccharide has a low degree of polymerization, coupled in reading frame to plant-specific transcription-initiation and termination signals.
A method for producing oligosaccharides having a low degree of polymerization, comprising the steps of:
A method for producing a plant having an increased proportion of oligosaccharides having a low degree of polymerization as compared to the plant in its natural state, comprising the steps of:
Dependencies are inferred from the claim text.