Quiescent cell populations for nuclear transfer in the production of non-human mammals and non-human mammalian embryos
AssigneesRoslin Institute (Edinburgh), The Minister of Agriculture, Fisheries & Food, Biotechnology & Biological Sciences Research Council
InventorsKeith Henry Stockman Campbell, Ian Wilmut
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Abstract
A method of reconstituting a mammalian embryo involves transferring the nucleus from a quiescent donor cell into a suitable recipient cell. The donor cell is quiescent, in that it is caused to exit from the growth and division cycle at G1 and to arrest in the G0 state. Nuclear transfer may take place by cell fusion. The reconstituted embryo may then give rise to one or more mammals. The invention is useful in the production of transgenic mammals as well as non-transgenics of high genetic merit.
Claims23
A method of reconstituting a non-human mammalian embryo, comprising
- a. transferring the nucleus of a quiescent diploid donor cell into a suitable enucleated recipient cell of the same species, thereby obtaining a reconstituted cell;
- b. activating the recipient cell before, during or after nuclear transfer;
- c. incubating the reconstituted cell such that an embryo develops.
Dependencies are inferred from the claim text.