Corticotropin releasing factor receptor 1-deficient mice

A method of producing a transgenic mouse in whose genome one or both alleles of the corticotropin releasing factor receptor-1 gene have been disrupted, said method comprising:

• a) producing positive ES cells by introducing a corticotropin releasing factor receptor-1 transgene derived from a mouse corticotropin releasing factor receptor-1 gene into embryonic stem cells, said transgene comprising a gene encoding a selectable marker in place of exon 5 through exon 8 of said corticotropin releasing factor receptor-1 gene, wherein ES cells that survive and grow under selection for said selectable marker are positive ES cells;
• b) introducing said positive ES cells into C57BL/6 blastocysts;
• c) transferring said blastocysts into pseudopregnant mice;
• and
• d) identifying transgenic progeny in which one or both alleles of the endogenous corticotropin releasing factor receptor-1 gene have been disrupted.