Recombinant disulfide-stabilized polypeptide fragments having binding specificity
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsIra H. Pastan, Byungkook Lee, Sun-Hee Jung, Ulrich Brinkmann
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Abstract
The present invention relates to disulfide-stabilized recombinant polypeptide molecules which have the binding ability and specificity for another peptide, such as the variable region of an antibody molecule. Methods of producing these molecules and nucleic acid sequences encoding these molecules are also described. In particular, the invention discloses Fv antibody fragments stabilized by a disulfide bond connecting the V.sub.H and V.sub.L regions of the Fv fragment. The .alpha. and .beta. chains of T cell receptors may be similarly stabilized by means described in the invention.
Claims8
A nucleic acid that encodes a light chain variable region (V.sub.L) of an antibody wherein the V.sub.L contains a cysteine at position 42, 43, 44, 45, 46, 98, 99, 100, or 101, such positions being determined in accordance with the numbering scheme published by Kabat and Wu.
A nucleic acid that encodes a heavy chain variable region (V.sub.H) of an antibody wherein the V.sub.H contains a cysteine at position 43, 44, 45, 46, 47, 103, 104, 105 or 106, such positions being determined in accordance with the numbering scheme published by Kabat and Wu.
Dependencies are inferred from the claim text.