Cloning and sequencing of allergens of dermatophagoides (house dust mite)
AssigneeImmuLogic Pharmaceutical Corporation
InventorsWayne Robert Thomas, Geoffrey A. Stewart, Keven J. Turner, Richard J. Simpson
Life of this patent
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Abstract
Isolated DNA encoding allergens of Dermatophagoides (house dust mites) particularly of the species Dermatophagoides farinae and Dermatophagoides pteronyssinus, which are protein allergens or peptides which include at least one epitope of the protein allergen. In particular, DNA encoding two major D. farinae allergens, Der f I and Der f II and DNA encoding a D. pteronyssinus allergen, Der p I. In addition, the proteins or peptides encoded by the isolated DNA, their use as diagnostic and therapeutic reagents and methods of diagnosing and treating sensitivity to house dust mite allergens.
Claims12
An isolated DNA encoding a Der p I protein allergen, said DNA comprising the cDNA insert of phage clone .lambda.gt11 p1(13T), ATCC Deposit No. 69338.
An isolated DNA encoding a Der p I protein allergen, said DNA comprising the nucleotide sequence shown in FIG. 7 or the complementary nucleotide sequence thereto, or the coding region of the nucleotide sequence shown in FIG. 7.
An isolated DNA encoding a protein allergen of Dermatophagoides, said DNA comprising a nucleotide sequence which hybridizes under high stringency conditions to the complement of the nucleotide sequence shown in FIG. 7, wherein said high stringency conditions comprise hybridization at Tm-20 followed by at least one post-hybridization wash at Tm-12.
An isolated DNA encoding a peptide of a Der p I protein allergen, said DNA comprising a portion of the cDNA insert of phage clone .lambda.gt11 p1(13T), ATCC Deposit No. 69338, wherein said portion encodes at least one epitope.
An isolated DNA comprising a nucleotide sequence which encodes a peptide comprising at least one epitope from a Der p I protein allergen, said Der p I protein allergen comprising the amino acid sequence shown in FIG. 7.
An isolated DNA comprising a nucleotide sequence which encodes a peptide comprising at least one epitope from a Dermatophagoides protein allergen, said DNA comprising a nucleotide sequence which hybridizes under high stringency conditions to a portion of the complement of the nucleotide sequence shown in FIG. 7, wherein said portion encodes at least one epitope, and said high stringency conditions comprise hybridization at Tm-20 followed by at least one post-hybridization wash at Tm-12.
An isolated DNA encoding a Der p I protein allergen comprising the amino acid sequence shown in FIG. 7 or the mature portion thereof.
Dependencies are inferred from the claim text.